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Separation and extraction method of soil humus

An extraction method and technology for humic substances, which are applied in the field of humic substance biochemistry research, can solve the problems of hydrolysis and oxidation of some organic substances, and achieve the effects of simple operation, avoiding changes in chemical structure and properties, and convenient correlation.

Active Publication Date: 2022-04-29
HEILONGJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for the separation and extraction of soil humus in order to solve the technical problem of hydrolysis and oxidation of some organic matter caused by the separation and extraction of existing humus

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  • Separation and extraction method of soil humus
  • Separation and extraction method of soil humus
  • Separation and extraction method of soil humus

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specific Embodiment approach 1

[0021] Specific embodiment one: the separation and extraction method of a kind of soil humus in this embodiment: the soil sample is dissolved in the mixture of acetone and hydrochloric acid, filtered, and the filtrate is diluted with distilled water to obtain the precipitation of humic acid, and the supernatant is treated with hydrogen The sodium oxide solution was titrated to obtain the precipitate of humin, then the humic acid and humin were purified separately, and finally freeze-dried to obtain the dry matter of humic acid and humin.

specific Embodiment approach 2

[0022] Specific embodiment two: the difference between this embodiment and specific embodiment one is that the separation and extraction method of a kind of soil humus is carried out according to the following steps:

[0023] 1. Dissolve the soil sample in the mixed solution of acetone and hydrochloric acid, fully shake and dissolve, overnight, and take the filtrate after filtering;

[0024] 2. Dilute the filtrate to 2 times the original volume with distilled water, let stand overnight, centrifuge to obtain humic acid precipitate, titrate the supernatant to neutral with sodium hydroxide solution, let stand overnight, and centrifuge to obtain humin precipitate. Others are the same as the first embodiment.

specific Embodiment approach 3

[0025] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the mixture of acetone and hydrochloric acid described in step 1 is composed of acetone and hydrochloric acid with a mass concentration of 10% in a mass ratio of 9:1. Others are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a method for separating and extracting soil humus, relates to a method for separating and extracting humus, and belongs to the field of humus biochemical research. The invention aims to solve the technical problems of hydrolysis and oxidation of part of organic matters caused by existing humus separation and extraction. The extraction method comprises the following steps: dissolving a soil sample in a mixed solution of acetone and hydrochloric acid, filtering, diluting filtrate by using distilled water to obtain humic acid precipitate, titrating supernate by using a sodium hydroxide solution to obtain humin precipitate, then respectively purifying the humic acid and the humin, and finally freeze-drying to obtain dry substances of the humic acid and the humin. According to the method, soil humic acid and humin are separated and extracted successively by adopting distilled water dilution precipitation and sodium hydroxide titration methods for the first time, and changes of chemical structures and characters of humus are avoided to the greatest extent. A low-concentration sodium hydroxide solution is adopted to dissolve humic acid precipitate, and purification is performed through cation exchange resin, so that the original molecular structure and chemical composition of humic acid are retained to the maximum extent.

Description

technical field [0001] The invention relates to a method for separating and extracting humus, belonging to the field of humus biochemistry research. Background technique [0002] Humus is an important part of terrestrial ecosystems, accounting for 60% of the total soil organic matter. According to the solubility of humic substances in acid-base solution, it can be divided into three parts: humic acid, fulvic acid and humin. Humin is soluble in alkali solution, but insoluble in acid, fulvic acid is soluble in acid and alkali solution, humin is the humic part closely combined with minerals, and insoluble in acid-base solution. Due to the complexity of the source of the original transformed material, the differences in microbial communities, environmental parameters, transformation time, and the polydispersity of humic substances, it is difficult to clarify the molecular structure of humic substances. Humic substances are small molecules and other heterogeneous polymers assoc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08H7/00C07G99/00
CPCC08H6/00C07G99/00
Inventor 宋鸽波波夫 A.I.石峰
Owner HEILONGJIANG UNIV
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