Preparation method and application of long-acting microalgae biological hydrogen production system

A technology of biology and microalgae, which is applied in the field of preparation of microalgae biological hydrogen production system, can solve the problem that the cost and efficiency of microalgae hydrogen production are difficult to balance, reduce the demand for dissolved oxygen and light, and maintain hydrogen production activity , the effect of promoting consumption

Active Publication Date: 2022-04-29
TONGJI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The present invention adopts a concurrent culture mode to cultivate microalgae aggregates, which can realize long-term and high-efficiency hydrogen production at low cost, solves the problem that the cost and efficiency of microalgae hydrogen production are difficult to balance in the prior art, and is a kind of large-scale practical potential Microalgae Biological Hydrogen Production System

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  • Preparation method and application of long-acting microalgae biological hydrogen production system
  • Preparation method and application of long-acting microalgae biological hydrogen production system
  • Preparation method and application of long-acting microalgae biological hydrogen production system

Examples

Experimental program
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Effect test

Embodiment 1

[0042] This example provides a long-term preparation method of a microalgae biological hydrogen production system. It should be understood that this is only an example and not a limitation. The microalgae, liquid medium, and flocculant selected in this example , and organic carbon compounds, respectively Chlorella pyrenoidosa, TAP medium, cationic etherified starch, and glucose.

[0043] First use TAP medium to cultivate Chlorella pyrenoidosa, when the light absorption of the culture at 750nm reaches 0.3 (OD750=0.3), add glucose and cationic etherified starch to the Chlorella pyrenoidosa liquid culture to make it The aggregates of Chlorella pyrenoidosa were produced by flocculation under near-neutral pH conditions and entered into a mixed culture mode, and then gas chromatography was used to monitor the content of oxygen and hydrogen in the culture system. A gas chromatograph is used to monitor the change of oxygen and hydrogen content in the photobiological hydrogen productio...

Embodiment 2

[0054] Analysis of the effect of different glucose concentrations on the effect of CS flocculin Chlorella nuclei. A microplate reader was used to test the photometric absorption of the supernatant of the hydrogen production system prepared in Example 1 at 750 nm to analyze the flocculation of Chlorella pyrenoidosa in the system.

[0055] The result is as Figure 5 As shown, 12.5mM glucose can significantly enhance the photometric absorption at 750nm of the supernatant of the culture system of Chlorella pyrenoidosa without CS flocculation, and the photometric absorption of the supernatant at 750nm in the culture system adding CS, They are also higher than other culture systems flocculated by CS, which shows that 12.5mM glucose can promote the growth of Chlorella pyrenoidosa, thereby reducing the flocculation effect of limited amount of CS on Chlorella pyrenoidosa. Although 25mM glucose can also significantly enhance the photometric absorption at 750nm of the supernatant of Chl...

Embodiment 3

[0057] Observation and analysis of aggregates of Chlorella pyrenoidosa under mixed culture. The production of Chlorella pyrenoidosa aggregates in the hydrogen production system prepared in Example 1 and the size of the aggregates were observed and analyzed by an optical microscope.

[0058] The results of microscopic observations such as Figure 6 (The scale in the figure is 300 μm) As shown, after CS flocculation of Chlorella pyrenoidosa under combined culture, significant aggregates of Chlorella pyrenoidosa were produced in the original culture system. Among them, at the concentration of 50mM glucose, the aggregates of Chlorella pyrenoidosa formed most obviously in the culture system. The concentrations of 100mM, 25mM, and 12.5mM glucose followed successively. In addition, at the concentration of 12.5 mM glucose, the effect of CS flocculated Chlorella pyrenoidosa on forming aggregates was inferior to that of CS flocculated Chlorella pyrenoidosa aggregates formed under phot...

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Abstract

The invention provides a long-acting microalgae biological hydrogen production system preparation method, which comprises: S1, adding an organic carbon compound and a flocculant to a microalgae liquid culture with a pH value of 6-8, with the use amount of the organic carbon compound being 0.5-500 mM and the use amount of the flocculant being 0.1-100 g/L; and S2, then, sealing the culture system, and standing in an environment with the illumination intensity of 1000-5000 Lux and the temperature of 20-30 DEG C, so that the microalgae form aggregates, enter an overall anaerobic condition and continuously grow, thereby efficiently generating hydrogen for a long time. The microalgae aggregate is cultured in a mixotrophic mode, long-term efficient hydrogen production can be achieved at low cost, the problem that in the prior art, the cost and efficiency of microalgae hydrogen production are difficult to consider at the same time is solved, and the system is a microalgae biological hydrogen production system with large-scale practical potential.

Description

technical field [0001] The invention relates to the field of microalgae biological hydrogen production, in particular to a preparation method of a long-acting microalgae biological hydrogen production system. Background technique [0002] Hydrogen is regarded as an ideal fossil fuel replacement due to its advantages of zero carbon emission and high calorific value. However, the current commercial use of hydrogen in the market is still produced by traditional fossil energy such as natural gas, which requires a large amount of energy input and is accompanied by a large amount of carbon dioxide, so it is difficult to achieve net zero carbon emissions. In order to help realize the "double carbon" goal, it is very important to develop hydrogen production methods with low energy consumption and net zero carbon emissions. [0003] Biological hydrogen production uses renewable organisms as catalysts, which can catalyze hydrogen production under normal temperature and pressure condi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12N1/38C12N1/12C12R1/89
CPCC12P3/00C12N1/38C12N1/12
Inventor 陈杰柳华杰
Owner TONGJI UNIV
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