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Urine microalbumin detection kit and use method thereof

A technology of urine microalbumin and detection kit, which is applied in the directions of biological test, measuring device, material inspection product, etc., to achieve the effect of simple method

Pending Publication Date: 2022-04-29
SUZHOU BAIYUAN GENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] For this reason, the technical problem to be solved by the present invention is the existing daily inspection of urinary microalbumin, and then provides a urine microalbumin detection kit and its use method

Method used

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  • Urine microalbumin detection kit and use method thereof
  • Urine microalbumin detection kit and use method thereof
  • Urine microalbumin detection kit and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] This embodiment provides a preparation method of the kit, and the specific steps are as follows:

[0028] (1) Take 2mL of 0.1mol / L PBS buffer solution and 0.5mL of PEG-6000, add deionized water to make up to 20mL, mix well and divide into 50 parts on average, each 400μL of the first reagent;

[0029] (2) Take 0.3g of Brij-35, 0.3g of sodium azide and 0.3mL of goat anti-human MALB antibody, add deionized water to make up to 1mL, mix well and divide into 50 parts on average, each 20μL of the second reagent;

[0030] (3) Take 10mL of 0.1mol / L PBS buffer solution, add deionized water to make the volume to 100mL, and mix thoroughly to obtain a dilution;

[0031] (4) label the first reagent, the second reagent and the diluent obtained in the above steps, pack them into boxes, and attach instructions;

[0032] (5) Paste a seal on the obtained test kit.

Embodiment 2

[0034] This embodiment is the application of the kit obtained in Example 1, and measures 3 clinical urine samples. The specific steps are as follows:

[0035] (1) Prepare a series of urine microalbumin solutions of standard samples, the urine microalbumin concentrations are 10mg / L, 20mg / L, 30mg / L, 40mg / L, 50mg / L, 100mg / L, 150mg / L , 250mg / L;

[0036] (2) Take 80 μL of urine microalbumin solution of a series of standard samples and add 2 mL of diluent to dilute, then add 400 μL of the first reagent and 20 μL of the second reagent to fully react, use a spectrophotometer to read the absorbance value at 340nm, and the result As shown in Table 1 below:

[0037] The absorbance of the urine microalbumin solution of a series of standard samples of table 1

[0038]

[0039] Standard curve y=0.0021x+0.0024 can be obtained from Table 1, R 2 =0.999, where y is the absorbance, and x is the urine microalbumin concentration;

[0040] (3) Take 80 μL of clinical urine sample and add 2 mL...

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Abstract

The urine microalbumin detection kit comprises a first reagent, a second reagent and a diluent, the first reagent comprises a phosphate buffer salt solution and polyethylene glycol 6000, and the second reagent further comprises polyoxyethylene lauryl ether, sodium azide and a goat anti-human urine microalbumin antibody. The invention also discloses a use method of the kit. According to the kit, microalbumin in urine reacts with a goat anti-human anti-albumin antibody in a liquid phase to generate an mAlb antigen-antibody complex, then the content of the microalbumin in the urine is displayed through an absorbance value, the method is simple, meanwhile, the used reagents are common reagents, and daily inspection of the microalbumin in the urine is facilitated; meanwhile, the kit is high in accuracy and good in stability when being used for detecting microalbuminuria, and is suitable for large-scale use.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a urine microalbumin detection kit and a use method thereof. Background technique [0002] At present, the number of people suffering from diabetes, hypertension, and cardiovascular and cerebrovascular diseases is increasing day by day. With the development of the disease course, the possibility and degree of kidney involvement are also increasing. Usually, when the measured values ​​of blood urea and serum creatinine are normal, the damage to the kidneys cannot be ruled out, and when the results of the urine routine protein test are positive, the damage to the kidneys has often reached an irreversible stage, so the determination of urinary microalbumin has become more severe. It is increasingly used as an early diagnostic indicator of renal tissue damage. [0003] Urinary microalbumin is a diagnostic indicator of a large number of diseases, and timely t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/75G01N33/536G01N33/68
CPCG01N21/31G01N21/75G01N33/536G01N33/68
Inventor 车团结徐红张鑫衍李春沈颂东张莹
Owner SUZHOU BAIYUAN GENT CO LTD
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