Inbound plant quarantine virus identification method based on nanopore sequencing and application

Abstract

The invention provides an imported plant quarantine virus identification method based on nanopore sequencing and application, and the method comprises the following steps: firstly constructing an imported plant quarantine virus genome database, then detecting a to-be-detected sample by using a nanopore sequencing technology, and performing pretreatment to obtain analysis data; the analysis data is subjected to virus type identification on the basis of an imported plant quarantine virus genome database, a detection report for the to-be-detected sample is obtained, and the plant virus quarantine requirement can be met through a faster, more accurate and more comprehensive detection technology.

Description

technical field [0001] The invention relates to the field of plant virus quarantine, in particular to a nanopore sequencing-based identification method and application of imported plant quarantine viruses. Background technique [0002] Plant viruses are known as plant "cancers". According to incomplete statistics, the annual agricultural losses caused by plant viruses in the world account for 10% of the total output of food crops, and the losses are as high as 20 billion US dollars. With the frequent international agricultural exchanges, such as the transfer of seeds and seedlings, and the trade of fruits and vegetables, some quarantine plant viruses are likely to be introduced into my country. At present, our country pays more and more attention to national biological security, and puts forward higher requirements for plant quarantine. Therefore, the quarantine department urgently needs to improve the quarantine identification technology of plant viruses and strengthen the ...

AI Technical Summary

Problems solved by technology

However, virus detection based on NGS sequencing technology still faces many problems: 1. In terms of equipment, NGS sequencers are all large-scale instruments, which need to be installed in professional laboratories or company platforms; 2. In terms of experimental operations, the preparation of sequencing libraries is complicated and the steps It is cumbersome and requires professional experimental operators; 3. In the sequencing process and detection cycle, the experimental operation and sequencing take 2-3 days; 4. In terms of sequencing data quality and data analysis, since NGS relies on PCR amplification, Therefore, there is a certain sequencing preference, resulting in incomplete genome coverage; and because the NGS sequencing read length is less than Kb, the virus genome needs to be assembled, and the results are extremely dependent on the optimization and improvement of software and algorithms to a certain extent.

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