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Linked molecular marker combination for major sites of sucrose content of peanuts, primer composition, identification method and application of linked molecular marker combination and primer composition

A primer composition and molecular marker technology, applied in biochemical equipment and methods, microbial measurement/testing, recombinant DNA technology, etc., can solve problems such as limiting the progress of high-sucrose sweet peanuts

Active Publication Date: 2022-05-06
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few reports on sucrose content-related QTL loci or linked molecular markers in peanuts, which limits the progress of molecular marker-assisted breeding of high-sucrose sweet peanuts

Method used

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  • Linked molecular marker combination for major sites of sucrose content of peanuts, primer composition, identification method and application of linked molecular marker combination and primer composition
  • Linked molecular marker combination for major sites of sucrose content of peanuts, primer composition, identification method and application of linked molecular marker combination and primer composition
  • Linked molecular marker combination for major sites of sucrose content of peanuts, primer composition, identification method and application of linked molecular marker combination and primer composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Example 1. Acquisition of Peanut Sucrose Content-Linked Molecular Markers

[0082] 1. Test materials: The peanut variety "Zhonghua 16" was used as the female parent, and the germplasm resource material "Rwanda Peanut" was used as the male parent for hybridization, and a recombinant inbred line containing 242 lines was constructed by the single-seed method ( RIL) Group F 7 (2017), F 8 (2018), F 9 (2019) Generation.

[0083]2. Phenotype identification: From 2017 to 2019, "Zhonghua 16", "Rwanda Peanut" and F 7 (2017), F 8 (2018) and F 9 (2019) Generation of 242 strains from the RIL population. A completely randomized block design was used with at least two repetitions. Every time is to repeat each strain and plant 1 row of 12 plants, the row spacing is 30 centimeters, and the plant spacing is 20 centimeters. Adopt standard field management methods to harvest mature pods of 8-10 typical plants with normal growth. Select 15 to 20 plump, mature and non-spotted kernel...

Embodiment 2

[0093] Example 2. Verification of Peanut Sucrose Content-Linked Molecular Markers

[0094] Using the three molecular markers closely linked to the main QTL site qSCA06.2 for peanut sucrose content and two molecular markers closely linked to the main QTL site qSCB06.2 for peanut sucrose content obtained in Example 1, the pair "Zhonghua 16" and 242 F's of "Rwandan Peanuts" 10 (2021) strains were PCR amplified. Using the InDel marker SCM06-11, SCM06-12 or SCM06-13 primer pair to amplify the same band pattern as the female parent "Zhonghua 16", denoted as aa, and amplify the same band pattern as the male parent "Rwanda Peanut" All are recorded as AA (Advanced Allele). Analyzing the phenotypic data of the RIL population (2017-2019), it was found that the average sucrose content of kernels of aa genotype materials was 1.58±0.03%, and the average kernel sucrose content of AA genotype materials was 2.69±0.04%. The three-year data showed that having the qSCA06.2 excellent allele cou...

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Abstract

The invention belongs to the technical field of molecular genetic breeding of peanuts, and particularly relates to a molecular marker combination linked with major QTL (quantitative trait loci) qSCA06.2 and qSCB06.2 of the sucrose content of peanuts, a primer composition, an identification method and application of the molecular marker combination. According to the invention, the QTL sites qSCA06.2 and qSCB06.2 related to the sucrose content of peanuts and the linked molecular markers thereof are screened and disclosed for the first time, and the QTL sites can be effectively applied to high-sucrose breeding and screening of peanuts. The breeding efficiency is improved, and high-sucrose genetic improvement of peanuts is accelerated.

Description

technical field [0001] The invention belongs to the technical field of molecular genetic breeding of peanuts, and in particular relates to a combination of molecular markers linked to main QTL sites qSCA06.2 and qSCB06.2 for sucrose content in peanuts, a primer composition, an identification method and an application thereof. Background technique [0002] As an important oil crop and economic crop in my country, peanut (Arachis hypogaea L.) is an important source of edible vegetable oil and protein. Its seeds are rich in oil, protein, vitamins, resveratrol and other excellent mineral elements. Contains an average of about 1.5% sucrose. Peanuts in my country are mainly used for oil extraction and food processing, and the consumption of fresh peanuts is also increasing year by year. The sweetness of peanut kernels is mainly derived from sucrose (accounting for about 90% of the total sugar), and the sweetness is highly related to the taste quality of peanut kernels. When the su...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2600/13C12Q2531/113
Inventor 雷永廖伯寿王志慧晏立英陈玉宁淮东欣王欣康彦平
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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