Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

SNP molecular marker for detecting rice stripe resistant disease genes STV11 and application

A technique for resistance to stripe blight and molecular markers, applied in the field of molecular biology, can solve the problems of low breeding efficiency, long breeding cycle for phenotypic identification, and complicated operation.

Pending Publication Date: 2020-01-17
天津市农作物研究所
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of rice stripe leaf blight resistance STV11 The SNP molecular marker and application of the gene can solve the problems of long breeding period, high breeding cost and low breeding efficiency in the phenotypic identification of rice stripe leaf blight resistance
The invention also provides a method for detecting rice stripe leaf blight resistance STV11 Gene SNP molecular marker primer set and KASP method solve the problems of unsafe experimental operation, complicated operation and inaccurate manual identification results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • SNP molecular marker for detecting rice stripe resistant disease genes STV11 and application
  • SNP molecular marker for detecting rice stripe resistant disease genes STV11 and application
  • SNP molecular marker for detecting rice stripe resistant disease genes STV11 and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The application of resistance gene-linked SNP marker K_180405 in natural population materials was verified by selecting 46 natural population materials, including 37 japonica and 9 indica.

[0057] 1. DNA extraction method:

[0058] The DNA of each variety (line) was extracted by CTAB method. Take the leaves at the seedling stage, grind them into powder with liquid nitrogen, and quickly transfer them into a 1.5 mL Eppendorf tube; add 800 μL of CTAB (1% w / v CTAB, 0.7 M NaCl, 10 mM EDT, 50 mMTris-HCl, pH8.0) Mix the extraction buffer, put it in a water bath at 65°C for 45 minutes, shake it gently every 10 minutes, centrifuge at 12000 rpm for 10 minutes; carefully absorb the supernatant, add an equal volume of chloroform, mix well, let it stand for stratification, 12000 rpm, centrifuge for 10 min; carefully absorb the supernatant, add an equal volume of isopropanol, and put it in a -20°C refrigerator for precipitation for more than 1 h. Centrifuge at 12000 rpm for 10 min...

Embodiment 2

[0066] Application of resistance gene-linked SNP marker K_180405 in segregated populations of breeding materials.

[0067] 1. Detect the genotype of a single plant in the F2 segregation population of the cross between the donor parent Nanjing 9108 and the recipient parent Jinjing 818, the method is the same as in Example 1.

[0068] 2. Carry out field resistance identification of rice stripe leaf blight on parents and individual plants of F2 segregation population, and determine the disease resistance level according to the incidence rate. The incidence rate of resistance (R) is less than 15%, and the incidence rate of sensitivity (S) is high at 15%.

[0069] The results of fluorescence scanning of 94 test materials are as follows: figure 2 As shown, the specific typing results of the markers are shown in Table 4.

[0070] Table 4 Testing and phenotypic verification of the marker K_180405 in Jinjing 818´Nanjing 9108 segregation population

[0071] Table 4 F2 segregation po...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an SNP molecular marker for detecting rice stripe resistant disease genes STV11 and application and discloses an SNP marker K_180405 which is co-separated with the rice stripe resistant disease genes and has a good amplification effect. The marker is used for detecting No.11 chromosome-bit base of paddy rice, and the polymorphism is A / T. Based on a KASP technology, a sequence of the developed marker K_180405 primer is shown in SEQ ID No.1-4. A site of the STV11 detected by the SNP molecular marker disclosed by the invention is a site with high specificity, whether paddyrice samples contain the STV11 gene or not can be conveniently and effectively authenticated, and homozygous genotype and heterozygous genotype of the STV11 can be accurately distinguished. An application method of the SNP molecular marker provided by the invention is accurate, reliable, simple and convenient to operate, and is applied to authentication and assisted selective breeding of the ricestripe resistant disease genes STV11.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to rice stripe leaf blight resistance STV11 SNP molecular marker of gene and method for detecting the molecular marker. Background technique [0002] Rice is an important ration crop in my country. Rice stripe leaf blight is a viral disease transmitted by striatellus striatellus, which is mainly harmful to japonica rice. Since the 1990s, the disease has been prevalent in the middle and lower reaches of the Yangtze River for many years and caused huge losses to the japonica rice production in the region. Breeding disease-resistant varieties as the most economical and effective way can fundamentally control the damage caused by rice stripe leaf blight. Major QTL for stripe leaf blight resistance from indica rice variety Modan in Jiangsu Province ( stv-b ) carried out MAS work, and bred a batch of resistant materials with good application effect. but due to stv-b It h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2563/107C12Q2535/125
Inventor 王晓静孙林静孙玥闫双勇张融雪李军玲
Owner 天津市农作物研究所
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com