Construction method and application of Tecrl knockout mouse model

A technology of mouse model and construction method, applied to other methods of inserting foreign genetic materials, chemical instruments and methods, biochemical equipment and methods, etc.
CN114480508APending Publication Date: 2022-05-13SHANGHAI CHILDRENS HOSPITAL
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI CHILDRENS HOSPITAL
Publication Date
2022-05-13

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Abstract

The invention provides a construction method and application of a Tecrl knockout mouse model, and the construction method of the Tecrl knockout mouse model comprises the following steps: A, designing sgRNA aiming at a Tecrl gene according to a gene knockout target; and B, injecting Cas9mRNA and sgRNA into a fertilized egg of an experimental mouse at the same time to obtain an F0-generation mouse of which the Tecrl gene is successfully knocked out. According to the invention, a Tecrl knockout mouse model is constructed for the first time, and it is found that the congenital mouse gene defect causes damage to the systolic and diastolic functions of the mouse in the early stage, so that a model is provided for researching the left heart function. The Tecrl knockout mouse has polymorphic and bidirectional ventricular tachycardia under induction of epinephrine and caffeine, clinical manifestations of CPVT patients in a movement or pressure state are simulated clinically, and a powerful tool is provided for studying the pathophysiological mechanism of catecholamine sensitive ventricular tachycardia.
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Description

technical field

[0001] The invention relates to the technical field of mouse model construction, in particular to a method for constructing a Tecrl knockout mouse model and its application. Background technique

[0002] Sudden cardiac death caused by arrhythmia is the leading cause of death in patients without cardiac organic changes. Catecholamine-sensitive ventricular tachycardia (CPVT) is a hereditary ion channelopathy, which mainly occurs in children aged 7-12, with an incidence rate of about 1 / 10000. Its main clinical feature is polymorphic or bidirectional ventricular tachycardia induced by exercise or emotional agitation. The first symptom of some patients with catecholamine-sensitive VT is syncope or sudden death. However, the specific pathogenesis is still unclear.

[0003] The diagnosis of catecholamine-sensitive ventricular tachycardia mainly depends on the characteristic electrocardiogram and genetic testing. For patients with typical CPVT, ECG exercise stres...

Claims

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