Liquid chromatography-tandem mass spectrometry detection method for fat-soluble vitamins based on magnetic solid-phase extraction
A technology of fat-soluble vitamins and tandem mass spectrometry, which is applied in the field of liquid chromatography-tandem mass spectrometry detection of fat-soluble vitamins, can solve the problems that are not conducive to improving the work efficiency of clinical mass spectrometry sample pretreatment links, and it is not convenient for pretreatment, so as to reduce instrument pollution Risk, increase the adsorption rate and enrichment effect, reduce the effect of variability
Pending Publication Date: 2022-05-13
天津国科医疗科技发展有限公司
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Problems solved by technology
The above three methods also have a common problem, that is, it is not convenient to realize fully automated sample pretreatment, and it is not conducive to improving the work efficiency of clinical mass spectrometry sample pretreatment.
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Embodiment 1
[0075] This embodiment provides a liquid chromatography tandem mass spectrometry detection method for fat-soluble vitamins based on magnetic solid-phase extraction, the method comprising the following steps:
[0076] 1. Preparation of test samples:
[0077] 1-1) Take 100 μL of the fat-soluble vitamin sample to be tested, add 10 μL of internal standard solution to it, and mix at 1000 rpm for 2 minutes;
[0078] 1-2) Add 2.5 mg of magnetic solid-phase extraction filler to 200 μL of methanol for activation, and then transfer the magnetic solid-phase extraction filler to 400 μL of water for filler equilibrium by applying an external magnetic field;
[0079] 1-3) Add the magnetic solid-phase extraction filler to the solution obtained in step 1-1), and mix for 2 minutes to obtain a mixed solution A;
[0080] 1-4) Transfer the magnetic solid-phase extraction filler in the mixed solution A to the cleaning solution for cleaning by applying an external magnetic field, specifically incl...
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The invention discloses a liquid chromatography-tandem mass spectrometry detection method for fat-soluble vitamins based on magnetic solid-phase extraction, which comprises the following steps: 1) preparing a test sample; 2) constructing a standard curve; and 3) detecting the test sample by adopting a liquid chromatography-tandem mass spectrometry method. According to the liquid chromatography-tandem mass spectrometry detection method for the fat-soluble vitamins based on the magnetic solid-phase extraction, provided by the invention, the fat-soluble vitamins in a sample are pre-treated by using a magnetic solid-phase extraction method, and a magnetic solid-phase extraction filler is transferred by virtue of an external magnetic field based on matrix dispersed solid-phase extraction; the whole experiment process is easy to realize automation; meanwhile, the advantages of high selectivity, high purification rate and the like of solid-phase extraction are reserved; the contact area is increased through dispersion and adsorption of the magnetic solid-phase extraction filler, the adsorption rate and the enrichment effect can be improved, and the balance time is shortened.
Description
technical field [0001] The invention relates to the technical field of mass spectrometry detection, in particular to a liquid chromatography tandem mass spectrometry detection method for fat-soluble vitamins based on magnetic solid-phase extraction. Background technique [0002] Vitamin A is a fat-soluble vitamin that plays an important role in human growth and development, maintaining normal body functions, and resisting diseases. Retinyl esters, which are composed of retinol and long-chain fatty acids, are the main form of vitamin A stored in the human body. Lack of vitamin A can lead to night blindness, impaired reproductive function, decreased immune function, abnormal bone metabolism and other physiological damage; and excessive intake of vitamin A can cause toxic and side effects. Vitamin D is a fat-soluble vitamin, mainly composed of two active ingredients, vitamin D2 and vitamin D3. Vitamin D is converted into 25-hydroxyvitamin D in the liver. Clinically, 25-hydroxyv...
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Login to View More IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/027G01N2030/062
Inventor 覃素姿李艳杰曹佳利赵勇周玉松周传贵李艳胡玮程文播
Owner 天津国科医疗科技发展有限公司