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Chemiluminescence immunoassay kit for free IgE (Immunoglobulin E)

A chemiluminescence immunoassay and detection kit technology, which is applied in the field of free IgE chemiluminescence immunoassay kits, can solve the problems of anti-IgE antibody detection, inability to distinguish free IgE and total IgE, and achieve good stability, high accuracy, The effect of high sensitivity

Pending Publication Date: 2022-05-13
长沙海柯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, domestic and foreign literatures do not describe in detail the detection methods of free IgE and total IgE levels in serum, and commercially available kits cannot distinguish free IgE and total IgE, which causes great troubles in the preclinical evaluation of anti-IgE antibodies

Method used

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  • Chemiluminescence immunoassay kit for free IgE (Immunoglobulin E)
  • Chemiluminescence immunoassay kit for free IgE (Immunoglobulin E)
  • Chemiluminescence immunoassay kit for free IgE (Immunoglobulin E)

Examples

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Embodiment 1

[0073] This embodiment provides a free IgE chemiluminescence immunoassay kit, which includes R1 reagent, R2 reagent and R3 reagent.

[0074] The composition of R1 reagent:

[0075]

[0076]

[0077] The preparation method of R1 reagent:

[0078] Labeling process of biotin-labeled FcεRI / FcεRIA protein: Take 440 μL DMSO and add it to a 2 mg active biotin bottle, mix well, then take 1 mg of FcεRI / FcεRIA to be labeled in an ultrafiltration tube, and add an appropriate volume of labeling buffer Set the volume to 0.5mL so that the final concentration is 2mg / mL. After mixing, centrifuge in a high-speed refrigerated centrifuge at a speed of 12000r / min at a temperature of 2-8°C for 10 minutes. After centrifugation, add 33.3μL Biotin solution and 466.7 μL labeling buffer, and mix by gently pipetting. Place in a 37°C incubator and incubate in the dark for 30 minutes, centrifuge after incubation at 12,000 r / min at a temperature of 2-8°C for 10 minutes. After centrifugation, add 5...

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Abstract

The invention provides a chemiluminescence immunoassay kit for free IgE (Immunoglobulin E). The free IgE chemiluminescence immunoassay kit comprises a reagent R1, a reagent R2 and a reagent R3, the reagent R1 is a buffer solution containing biotin-labeled Fc epsilon RI / Fc epsilon RIA protein and a first protective agent, and the first protective agent comprises casein, animal serum and bovine serum albumin L; the R2 reagent is a buffer solution containing an anti-human IgE antibody marked by a luminous marker and a second protective agent, and the second protective agent comprises casein and animal serum; and the R3 reagent is a buffer solution containing streptavidin-labeled magnetic microparticles. The kit has the advantages of good stability, high accuracy, high sensitivity and the like.

Description

technical field [0001] The invention relates to the technical field of antibody detection, in particular to a free IgE chemiluminescence immunoassay kit. Background technique [0002] Allergic asthma is a chronic inflammatory respiratory disease caused by the combination of inhaled antigen and specific IgE. After the complex formed by the combination of inhaled antigen and IgE is cross-linked with high-affinity receptors on the surface of effector cells such as mast cells and basophils, inflammatory mediators can be released, thereby causing airway contraction and inflammatory response. Omalizumab, a humanized anti-IgE monoclonal antibody, is the world's first innovative drug for the treatment of asthma and is used for the treatment of moderate to severe persistent allergic asthma. Omalizumab inhibits the binding of IgE to the IgE receptor (FcεRI) on the surface of mast cells and basophils by binding to the Fc fragment of IgE molecule Cε3, thereby preventing the degranulati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/543G01N21/76
CPCG01N33/6854G01N33/54326G01N21/763G01N2800/24
Inventor 石慧
Owner 长沙海柯生物科技有限公司