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Automatic cell preparation device

A technology for preparing devices and cells is applied in the field of automated cell preparation devices to achieve the effects of simple control, low cost, and simple and reliable rotating structure

Pending Publication Date: 2022-05-24
浙江泰林医学工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the problems caused by manual operation in the aseptic cell preparation process in the prior art, the present invention provides an automated cell preparation device, the entire cell preparation process is completed in the isolator, and the simple and repeated liquid replacement work is automated Instead, the entire liquid change process is fully automatic, reducing the risk of operational errors and contamination caused by manual operations

Method used

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  • Automatic cell preparation device
  • Automatic cell preparation device
  • Automatic cell preparation device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] like figure 1 As shown, an automated cell preparation device includes: a sterile operation chamber 15, a sterile transfer chamber 16, an automatic liquid exchange device 18 and a cell incubator 17; Environment, the aseptic operation cabin 15 is provided with an embedded refrigerator 15.1, a waste channel 15.2, an embedded centrifuge 15.3, a planktonic bacteria sampler 15.4 and a particle sampler 15.6; the aseptic transfer cabin 16 is used for testing reagents , The material is transferred from the sterile environment to the sterile aseptic operation cabin 15; the automatic liquid exchange device 18 is used for automatic liquid exchange of the cell culture medium, and the aseptic operation cabin 15 is set in the aseptic transfer cabin 16 and fully automatic. Between the liquid exchange devices 18; the cell incubator 17 is used for culturing cells, and the fully automatic liquid exchange device 18 is arranged between the aseptic operation cabin 15 and the cell incubator 1...

Embodiment 2

[0036] like figure 2 As shown, on the basis of Embodiment 1, the fully automatic liquid exchange device 18 includes a fixed table 1, a transfer manipulator 2, a liquid exchange mechanism 3, a petri dish worktable 4, a needle placement rack 6, and the needle placement rack 6 is provided with There are several unused suction needles 3.1.5, and several unused suction needles 3.1.5 are neatly arranged; the transfer manipulator 2 is used for the petri dish body 5, and the transfer manipulator 2 is set on the fixed table 1; image 3 As shown, the transfer manipulator 2 includes a translation mechanism 2.1, a lifting mechanism 2.2, a rotating mechanism 2.3 and a transfer plate 2.4, the transfer plate 2.4 is fixed on the rotating mechanism 2.3, the rotating mechanism 2.3 is arranged on the lifting mechanism 2.2, and the lifting mechanism 2.2 is fixed on the On the translation mechanism 2.1, the translation mechanism 2.1 and the lifting mechanism 2.2 are rack and pinion mechanisms dri...

Embodiment 3

[0040] like figure 2 and Figure 4 As shown, on the basis of Embodiment 2, a waste needle collection box 7 is arranged on the fixing table 1 , and a needle removal block 8 is fixed above the waste needle collection box 7 . like Figure 7 As shown, the needle removal block 8 is provided with a limit groove 8.1 adapted to the suction needle 3.1.5, and a positioning column 13 is provided around the waste liquid discharge bucket 12, and the positioning column 13 is fixed with the fixing table 1 . A snap ring 3.1.4 is provided on the outer side wall of the liquid aspiration needle 3.1.3, and the liquid aspiration needle 3.1.3 is clamped to the liquid aspiration needle 3.1.5 on the liquid aspiration needle 3.1.3 through the snap ring 3.1.4. A waste needle collection box 7 is arranged on the fixing table 1, and a needle removal block 8 is fixed above the waste needle collection box 7. The needle removal block 8 is provided with a limit groove 8.1 adapted to the suction needle 3.1....

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PUM

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Abstract

The invention discloses an automatic cell preparation device which comprises a sterile operation cabin used for providing a sterile environment for cell extraction operation; the sterile transfer cabin is used for transferring test reagents and materials into the sterile operation cabin from a sterile environment; the full-automatic liquid changing device is used for automatically changing the liquid of the cell culture medium, and the sterile operation cabin is arranged between the sterile transfer cabin and the full-automatic liquid changing device; the cell culture box is used for culturing cells, and the full-automatic liquid changing device is arranged between the sterile operation cabin and the cell culture box. According to the automatic cell preparation device provided by the invention, the whole cell preparation process is completed in the isolator, and simple and repeated liquid change work is replaced by automation, so that full-automatic liquid change is realized in the whole liquid change process, and the risks of operation errors and pollution caused by manual operation are reduced.

Description

technical field [0001] The invention relates to the technical field of cell liquid exchange equipment, in particular to an automated cell preparation device. Background technique [0002] At present, cell preparation operations are mostly carried out in open cell laboratories, mainly manual operations, and cell preparation personnel need to be trained. Problems such as contamination risk and poor reproducibility caused by manual operation affect the quality of cell preparation. If automation can be used to replace manual labor in the process of cell exchange, the risk of cell exchange contamination will be reduced, and the exchange efficiency will be greatly improved. Therefore, it is necessary to provide an automated cell preparation device. The entire cell preparation process is completed in the isolator, which meets the requirements of GMP, so that the entire liquid exchange process can realize fully automatic liquid exchange, and reduce the operation error and pollution...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/36C12M1/34C12M1/26C12M1/22C12M1/12C12M1/10C12M1/00
CPCC12M23/10C12M23/48C12M23/52C12M29/00C12M33/00C12M37/00C12M37/04C12M41/36C12M41/48C12M47/04C12M47/20
Inventor 陈军峰赵振波夏信群叶大林
Owner 浙江泰林医学工程有限公司