Surface molecular imprinting gel photonic crystal sensor as well as preparation method and application thereof

A surface molecular imprinting and photonic crystal technology, applied in the field of analysis and detection, can solve the problems of difficult fixation of target molecules and lack of functional groups in colloidal particles, and achieve the effect of rapid naked-eye detection

Pending Publication Date: 2022-05-24
WUXI VOCATIONAL & TECHN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the micelles used in the preparation of molecularly imprinted gel photonic crystal sensors lack functional groups, it is difficult to immobilize target molecules on them, making the preparation of surface molecularly imprinted gel photonic crystal sensors still challenging.

Method used

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  • Surface molecular imprinting gel photonic crystal sensor as well as preparation method and application thereof
  • Surface molecular imprinting gel photonic crystal sensor as well as preparation method and application thereof
  • Surface molecular imprinting gel photonic crystal sensor as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Preparation of polystyrene-acrylic acid colloidal crystals (hereinafter referred to as PSA colloidal crystals)

[0041] Preparation of PSA microsphere emulsion: The soap-free emulsion polymerization method was used to prepare the PSA micelle emulsion. The mass fraction of the PSA micelle emulsion was 7.3%, the particle size was 200 nm, and the monodispersity coefficient was <0.005.

[0042] Preparation of PSA colloidal crystals: Dilute the prepared PSA microsphere emulsion to 1% to obtain emulsion A. Dissolve 0.1 mg of tetracycline in emulsion A to prepare emulsion B; then put a hydrophilic glass sheet vertically into emulsion B, place it in a constant temperature and humidity chamber at a temperature of 60 °C and a humidity of 40% for 24 h, and place it on PSA colloidal crystals with tetracycline evenly distributed on the surface of the colloidal particles were prepared on the chip.

[0043] 2. Fabrication of Surface Molecularly Imprinted Gel Photonic Crystal Senso...

Embodiment 2

[0050] The difference from Example 1 is that when preparing PSA colloidal crystals, the quality of tetracycline added is 0.5 mg; when preparing the precursor solution, 7 mmol of acrylamide (hereinafter referred to as AM) and 3.5 mmol of itaconic acid (hereinafter referred to as IA), 0.42 mmol N,N-methylenebisacrylamide (hereinafter referred to as BIS) was used as the cross-linking agent, and 0.0035 mmol ammonium persulfate (hereinafter referred to as APS) was used as the initiator. Finally, the surface molecularly imprinted gel photonic crystal sensor was prepared.

Embodiment 3

[0052] The difference from Example 1 is that when preparing PSA colloidal crystals, the quality of tetracycline added is 1 mg; when preparing the precursor solution, 14 mmol of acrylamide (hereinafter referred to as AM) and 3.5 mmol of itaconic acid (hereinafter referred to as IA), 0.7 mmol N,N-methylenebisacrylamide (hereinafter referred to as BIS) was used as the cross-linking agent, and 0.0035 mmol ammonium persulfate (hereinafter referred to as APS) was used as the initiator. Finally, the surface molecularly imprinted gel photonic crystal sensor was prepared.

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Abstract

The invention relates to a surface molecular imprinting gel photonic crystal sensor as well as a preparation method and application thereof. The prepared surface molecular imprinting gel photonic crystal sensor can be used for rapid qualitative and quantitative detection of target molecules. According to the invention, a colloidal crystal formed by simultaneous self-assembly of tetracycline and polystyrene-acrylic acid (PSA) colloidal particles is used as a template, and acrylamide and itaconic acid are used as bifunctional monomers to polymerize in gaps of the colloidal crystal to prepare the surface molecular imprinting gel photonic crystal sensor. The obtained sensor is fast in response to target molecules (lt; 60 s), the Bragg diffraction wavelength is subjected to red shift along with the increase of the concentration of the target compound in the solution, and obvious color change can be observed by naked eyes. The red shift amount (delta lambda) and the negative logarithm (-logC) of the target molecule concentration are in a linear relationship, so that the sensor can be used for quantitative detection. The sensitivity and the detection speed of the molecularly imprinted gel photonic crystal sensor are improved.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a surface molecularly imprinted gel photonic crystal sensor and its preparation method and application. Background technique [0002] Molecularly imprinted polymers with specific molecular recognition sites have become one of the most competitive artificial receptors due to their ease of synthesis, low cost, high stability, and high specific binding ability. Therefore, molecularly imprinted polymer-based sensors, such as electrochemical sensors, quartz crystal microbalance sensors, and gel photonic crystal sensors, exhibit high selectivity, excellent stability, and long lifetime. Unlike other sensors, molecularly imprinted gel photonic crystal sensors can directly convert molecular recognition behavior into visually perceivable color changes due to the unique photonic bandgap, thereby enabling rapid detection of target molecules without any instrument, ...

Claims

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Application Information

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IPC IPC(8): G01N23/2251G01N23/2202
CPCG01N23/2251G01N23/2202
Inventor 杨兆昆黄士宇秦立彦
Owner WUXI VOCATIONAL & TECHN COLLEGE
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