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Three-method synergistic extraction process of laminarin

A technology of laminar polysaccharide and extraction process, which is applied in the field of three-method collaborative extraction process of laminar polysaccharide, can solve the problem that polysaccharide cannot meet the demand of polysaccharide, achieve the effect of improving utilization rate, safe and reliable extraction method, and improving yield

Active Publication Date: 2022-05-27
JIANGNAN UNIV
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  • Application Information

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Problems solved by technology

However, the yield of polysaccharides obtained by extraction methods such as hot water, acid, and alkali extraction methods, ultrasonic-assisted extraction methods, and enzyme extraction methods has not been able to meet people's needs for polysaccharides.

Method used

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  • Three-method synergistic extraction process of laminarin
  • Three-method synergistic extraction process of laminarin
  • Three-method synergistic extraction process of laminarin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 The three-method collaborative extraction process of kelp polysaccharide

[0032] Cut the kelp into pieces and mix it with water in a certain mass ratio (kelp: water = 1:10), add cellulase (1.5%), and then enzymatically extract it at a certain temperature (60°C), and after a certain time of extraction Add citric acid (citric acid: kelp pulp = 1:3-1:7) to the extraction solution by weight for ultrasonic extraction, neutralize the extraction solution to neutrality with potassium hydroxide, concentrate under reduced pressure, add five times the volume of anhydrous Ethanol, let stand at 4°C for 8h. The polysaccharide samples obtained by precipitation were redissolved in distilled water, deproteinized by Sevag method, and freeze-dried. Four kinds of kelp polysaccharide components were obtained by fractional alcohol precipitation operation. The crude polysaccharide yield is calculated as follows:

[0033]

Embodiment 2

[0034] Embodiment 2: do not add cellulase, fix the dosage ratio of citric acid and kelp pulp, the influence of different ultrasonic conditions on crude polysaccharide yield

[0035] Based on the process of Example 1, under the condition that no cellulase was added and the solid-liquid ratio was fixed (citric acid: kelp pulp=1:5), other conditions remained unchanged, the ultrasonic conditions were explored, respectively from ultrasonic power (240W -480W), ultrasonic temperature (40°C-80°C), and ultrasonic time (30min-150min) on the yield of crude polysaccharide were studied.

[0036] Depend on figure 1 (A) (B) (C) It can be seen that when the ultrasonic power is 420 W, the ultrasonic temperature is 60 °C, and the ultrasonic time is 90 min, the polysaccharide yield is the largest and the energy consumption is small.

[0037] Ultrasound can better destroy the structure of kelp tissue and release polysaccharides, but with the increase of ultrasonic power and the prolongation of u...

Embodiment 3

[0038] Embodiment 3: fixed ultrasonic condition, the consumption ratio of different citric acid and kelp pulp is on the influence of crude polysaccharide yield

[0039] Based on the process of Example 1, under the optimized ultrasonic conditions (ultrasonic power 420W, ultrasonic temperature 60°C, ultrasonic time 90min) obtained in Example 2 without adding cellulase, the ratio of solid to liquid (citric acid: kelp pulp) was changed. =1:3-1:7) to study the effect of crude polysaccharide yield.

[0040] The different citric acid of embodiment 3 and the consumption ratio of kelp pulp see the influence result for crude polysaccharide yield figure 1 (D). It was found that when the ratio of citric acid and kelp pulp was 1:4, the yield was the highest, reaching 10.43% ± 0.03%. Compared with the prior art, the acid extraction rate was only 7%, and the improvement was significant. The use of citric acid in the extraction process can provide an acidic environment, effectively degrade ...

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Abstract

The invention researches a novel three-method polysaccharide extraction process. Citric acid, ultrasonic waves and biological enzymes are used for extracting laminarin, and an L18 (37) orthogonal experiment method is adopted for optimizing all conditions. And separating the laminarin by adopting a graded alcohol precipitation method. Compared with the prior art, the method provided by the invention has the advantages that the polysaccharide yield of the laminarin is 17.93 + / -0.12%, which is superior to that of a common extraction method seen at present, the utilization rate and the polysaccharide yield of the laminarin raw material are greatly improved, and a new way is opened up for the extraction process of the polysaccharide. And the laminarin has biological activity and can become a new source of natural antioxidants.

Description

technical field [0001] The invention relates to the technical field of polysaccharide extraction, in particular to a three-method collaborative extraction process of kelp polysaccharide. Background technique [0002] Laminaria japomica, also known as kelp and cabbage, belongs to the Phaeophyta, Laminaria, Laminaceae, and Laminaria. In this paper, the extraction and exploration of kelp polysaccharide is carried out in order to explore the biological activity function of kelp raw materials, in order to better apply it in the field of functional food. [0003] The extraction of polysaccharides includes hot water extraction, acid and alkali extraction, ultrasonic-assisted extraction, and enzyme extraction. The hot water extraction method is simple and economical, but the extraction time is long, the polysaccharide extraction rate is low, and it does not utilize large-scale application production. Acid extraction and alkali extraction are beneficial to the release of polysaccha...

Claims

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Application Information

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IPC IPC(8): C08B37/00A23L33/125
CPCC08B37/0003A23L33/125A23V2002/00A23V2200/30
Inventor 谷怡静刘杰
Owner JIANGNAN UNIV
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