Detection method for rapidly distinguishing yersinia enterocolitica from yersinia intermediary

Abstract

The invention discloses a detection method for quickly distinguishing yersinia enterocolitica and yersinia intermediary, which is characterized in that a peptide fingerprint spectrum of the yersinia enterocolitica and the yersinia intermediary is detected by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry, and the yersinia enterocolitica and the yersinia intermediary can be distinguished according to different characteristic peaks of the two yersinia enterocolitica. And identifying the characteristic protein, and distinguishing the yersinia enterocolitica from the yersinia intermediary. According to the method, the identification time is shortened by 1-2 days compared with that of an existing traditional biochemical experiment, the yersinia enterocolitica and the yersinia intermediary can be rapidly distinguished, and rapid detection is achieved. The method is high in automation degree and short in detection time, the probability that front-line detection personnel make contact with the food-borne pathogenic bacteria is effectively reduced, and the risk that the food-borne pathogenic bacteria pollute people and the environment is reduced.

Description

technical field [0001] The invention relates to a method for rapidly distinguishing Yersinia enterocolitica from Yersinia intermedius by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry, MALDI-TOF MS) technology method for the detection of bacteria. Background technique [0002] Yersinia enterocolitica is widely distributed and can be found in raw vegetables, milk and dairy products, meat, soy products, salads, oysters, clams and shrimp. Also present in the environment, such as lakes, rivers, soil and vegetation. It has been isolated from the feces of livestock, dogs, cats, goats, squirrels, minks and primates. Around the harbour, many birds, including waterfowl and seagulls, may be carriers. It can cause a zoonotic disease called Yersiniosis, which can not only infect pigs, cattle, sheep and other animals, but also carry the bacteria in the feces of infected an...

AI Technical Summary

Problems solved by technology

PCR technology is a fast, sensitive, and specific technology, but at present, this technology still relies on the pre-enrichment step of the traditional method. The enrichment solution may contain PCR inhibitors, which will affect the PCR amplification results.

Both Yersinia enterocolitica and Yersinia intermedius belong to the genus Yersinia. It has been reported in the literature that the antigenicity of Yersinia is complex, and its antigenic properties are similar to those of other Enterobacteriaceae. Difficult to make specific identification between genus and species

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