Additive for preserving adipose-derived mesenchymal stem cells and application of additive
A technology of high-quality stem cells and additives, applied in the field of stem cells, can solve the problems of increased cost, protein denaturation, and high equipment requirements, and achieve the effects of safe ingredients, good proliferation activity, and stable storage environment
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0024] An additive for the preservation of adipose mesenchymal stem cells, the mass concentration of the additive in a low-sugar DMEM medium is: mannitol 2 mg / mL, silk fibroin 10 μg / mL, docusate sodium 8 μg / mL, glutathione 3 μg / mL mL, glycerol 0.8mg / mL, quercetin 12ng / mL.
[0025] The application of the above-mentioned additive for preservation of adipose-derived mesenchymal stem cells includes the following steps:
[0026] (1) adding additives to the culture medium, mixing to obtain a preservation solution, for subsequent use;
[0027] (2) Add the adipose-derived mesenchymal stem cells to be preserved in the preservation solution of step (1) to resuspend, and distribute them in 2 mL cryopreservation tubes. The density of adipose-derived mesenchymal stem cells in the preservation solution is 1×10 7 pcs / mL;
[0028] (3) Program cooling of the preservation solution in step (2) to -80°C, and the program cooling process is: cooling to -20°C at 1°C / min, and then cooling to -80°C ...
Embodiment 2
[0030] An additive for preservation of adipose mesenchymal stem cells, the mass concentration of the additive in a low-sugar DMEM medium is: mannitol 1 mg / mL, silk fibroin 7 μg / mL, docusate sodium 5 μg / mL, glutathione 1 μg / mL mL, glycerol 0.5mg / mL, quercetin 10ng / mL.
[0031] The application of the above-mentioned additive for preservation of adipose-derived mesenchymal stem cells includes the following steps:
[0032] (1) adding additives to the culture medium, mixing to obtain a preservation solution, for subsequent use;
[0033] (2) Resuspend the adipose-derived mesenchymal stem cells to be preserved with the preservation solution of step (1), and distribute them in 2 mL cryopreservation tubes. The density of adipose-derived mesenchymal stem cells in the preservation solution is 2×10 7 pcs / mL;
[0034] (3) Program cooling of the preservation solution in step (2) to -80°C, and the program cooling process is: cooling to -20°C at 2°C / min, and then cooling to -80°C at 18°C / mi...
Embodiment 3
[0036] An additive for preservation of adipose mesenchymal stem cells. mL, glycerol 1mg / mL, quercetin 15ng / mL.
[0037] The application of the above-mentioned additive for preservation of adipose-derived mesenchymal stem cells includes the following steps:
[0038] (1) adding additives to the culture medium, mixing to obtain a preservation solution, for subsequent use;
[0039] (2) Resuspend the adipose-derived mesenchymal stem cells to be preserved with the preservation solution of step (1), and distribute them in 2 mL cryopreservation tubes. The density of adipose-derived mesenchymal stem cells in the preservation solution is 3×10 7 pcs / mL;
[0040] (3) Program cooling of the preservation solution in step (2) to -80°C, and the program cooling process is as follows: cooling to -20°C at 3°C / min, and then cooling to -80°C at 20°C / min for preservation.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com