Bactericidal composition for preventing and treating ginger fusarium wilt
A composition and fusarium wilt technology, applied in the field of pesticides, can solve the problems of lack of effective control agents for ginger wilt, achieve the effects of reducing pesticide residues and environmental pollution, improving bactericidal virulence, and improving field control efficiency
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Embodiment 1
[0020] Example 1 Combined virulence of metconazole and tea tree essential oil against Fusarium solani
[0021] Metconazole was purchased from Adama Huifeng (Jiangsu) Co., Ltd., and tea tree essential oil was purchased from Jiangxi Wanhua Perfume Co., Ltd.
[0022] The mycelial growth rate method was used to determine the effect of metconazole and tea tree essential oil on Fusarium solani ( Fusarium solani ) indoor toxicity: according to the pre-test results, dilute the agent to 5-7 concentrations, mix it with PDA medium and pour it into a 9cm dish, and make a drug-containing plate after cooling, and add the same amount of sterilized water as the control , and then inoculate 5mm Fusarium rottennsis cake upside down in the center of the drug-containing plate, cultivate it at 28°C until the control plate is covered with 80% of the plate for measurement, measure the colony diameter by the cross method, calculate the growth inhibition rate and calculate the EC with the software 5...
Embodiment 2
[0026] Example 2 Synergistic effect of tea tree essential oil on metconazole
[0027] Choose a petri dish with a diameter of 9 cm, and paste a piece of filter paper with a diameter of 5 mm in the center of the lid of the dish, and use it after sterilization. On the ultra-clean workbench, pour 20 mL of PDA medium into the petri dish, mix with metconazole to make the final concentration 0.95 mg / L (theoretical growth inhibition rate is about 50%), and the remaining space volume in the dish is 70 mL. After inoculating the Fusarium bean curd cake with a diameter of 5mm, immediately add the final concentration of 0.095mg / L tea tree essential oil on the filter paper, and seal it quickly. Essential oil treatments served as controls, and each treatment was replicated 4 times. The cells were cultured at 28°C until the control plate covered 80% of the plate for measurement, and the colony diameter was measured by the cross method, and the growth inhibition rate was calculated.
[0028]...
Embodiment 3
[0030] Example 3 Field control effect of tea tree essential oil and metconazole on ginger fusarium wilt
[0031] Tea tree essential oil and metconazole are processed into 20% metconazole suspension, 1% tea tree essential oil microcapsule suspension, 21% metconazole + tea tree essential oil (20:1) suspension, 31% metconazole + tea tree essential oil (30:1) suspending agent, 41% metconazole + tea tree essential oil (40:1) suspending agent and 51% metconazole + tea tree essential oil (50:1) suspending agent for the control of ginger wilt in the field.
[0032] Choose a plot with severe ginger fusarium wilt, soak the ginger seeds before sowing the ginger seed blocks, and use 30 liters of water per mu. The field experiment was set up with 7 treatments, repeated 3 times, with clear water as the control, and the experimental plots were completely randomly arranged in blocks; the plots were 33.3 m2 in area 2 . Depending on the incidence of the Qingshui control area, the incidence of...
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