Method for constructing myocardial ischemia-reperfusion insulin resistance model by using primary myocardial cells

A technology for insulin resistance and cardiomyocytes, applied in the field of biomedicine, can solve the problems of high technical requirements for experimental operations, many interference factors in the body, and high economic costs, and achieve the effects of low economic costs, short experimental cycles, and few interference factors

Pending Publication Date: 2022-06-28
GUIZHOU MEDICAL UNIV
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  • Abstract
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Problems solved by technology

[0005] In order to solve the current experimental model for simulating insulin resistance of myocardial ischemia-reperfusion injury is an experimental animal model, and the animal model has technical defects and deficiencies such as high economic cost, long expe

Method used

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  • Method for constructing myocardial ischemia-reperfusion insulin resistance model by using primary myocardial cells
  • Method for constructing myocardial ischemia-reperfusion insulin resistance model by using primary myocardial cells
  • Method for constructing myocardial ischemia-reperfusion insulin resistance model by using primary myocardial cells

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Experimental program
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Embodiment 1

[0028] Experimental animals: 48 2-3d newborn SD rats (provided by the Experimental Animal Center of Guizhou Medical University).

[0029] Drugs and reagents: DMEM low-sugar culture medium, DMEM high-sugar culture medium, trypsin digestion solution, and PBS solution are all products of BasalMedia; newborn bovine serum is a product of Sijiqing company; 5-BrdU is a product of MCE company; 2-NBDG It is the product of Angekai Company; the insulin solution is the product of Proxa Corporation; the Glut4 antibody is the product of abcam Company.

[0030] The establishment method of primary cardiomyocyte model of oxygen-glucose deprivation insulin resistance, including primary cardiomyocyte culture, experimental grouping and modeling, identification of oxygen-glucose deprivation insulin-resistant cardiomyocyte model. The specific steps are:

[0031] Culture of primary cardiomyocytes: SD rats were taken from neonatal 1-3 days old, and the hearts were obtained by thoracotomy under steri...

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Abstract

According to the construction method for constructing the myocardial ischemia-reperfusion insulin resistance model by using the primary myocardial cells, the primary myocardial cells are obtained from an SD newborn suckling mouse, and an in-vivo myocardial ischemia-reperfusion experimental model is simulated through an experimental method of oxygen-glucose deprivation and reoxygenation. The model is low in economic cost, short in experimental period and few in interference factors, directly reflects pathophysiological changes of organ tissues, and has better stability compared with an animal model.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for constructing a myocardial ischemia-reperfusion insulin resistance model by using primary cardiomyocytes. Background technique [0002] Myocardial ischemia-reperfusion injury is one of the important pathophysiological mechanisms leading to cardiac dysfunction and even death. The currently recognized pathogenesis of myocardial ischemia-reperfusion injury includes oxygen free radical damage, calcium overload, programmed cell death, and myocardial insulin resistance. Myocardial insulin resistance is one of the initiating mechanisms of myocardial ischemia-reperfusion injury. In the pathological state of myocardial ischemia-reperfusion injury, the sensitivity of myocardial tissue and cells to physiological levels of insulin is reduced, resulting in disturbance of myocardial tissue energy metabolism, a series of serious metabolic complications, and irreversible damage ...

Claims

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Application Information

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IPC IPC(8): C12N5/077
CPCC12N5/0657C12N2509/00C12N2500/02C12N2500/34
Inventor 巫洪坤梁贵友宋颖楠潘斯斯王峰陈开远刘洲白珏高维龙惠永鹏
Owner GUIZHOU MEDICAL UNIV
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