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Semi-quantitative rapid detection method for parthenolide

A parthenolide and fast technology, applied in measuring devices, material analysis through observation of the influence on chemical indicators, instruments, etc., can solve the problems of inconvenient inspection and judgment, long time-consuming, high equipment configuration, etc.

Pending Publication Date: 2022-07-01
ACCENDATECH LUOYANG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of checking parthenolide in plants in the laboratory can be carried out by high-performance liquid chromatography external standard method, which requires high equipment configuration and takes a long time, which is extremely inconvenient when inspecting and judging plant growth or planting sites

Method used

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  • Semi-quantitative rapid detection method for parthenolide
  • Semi-quantitative rapid detection method for parthenolide
  • Semi-quantitative rapid detection method for parthenolide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Sample preparation: Take 10 parts of the root bark of Magnolia japonica, add it to a 500ml conical flask, add 100 parts of ethanol, soak at 25-30°C for 2 hours, shake well to get the sample solution for later use.

[0025] Preparation of standard solution: take parthenolide reference substance and prepare standard solution with 0.2% and 0.1% content.

[0026] Detection: Using a 2ul quantitative capillary, take the same volume of sample solution and standard solution, respectively, and point them on the thin-layer chromatography; use ethyl acetate to develop, after completion, dry the thin-layer chromatography plate and put it in iodine / silica gel for 5 minutes , remove the observation spot.

[0027] Conclusion: by attaching figure 1 The results showed that the sample spots and the reference substance spots appeared in the same position, indicating that they contained the target compound; the color of the sample spots was darker than that of the 0.1% standard solution, ...

Embodiment 2

[0029] Sample preparation: Take 10 parts of the dried residue of the root bark of Magnolia japonica after extraction, add it to a 500ml conical flask, add 200 parts of ethanol, soak at 25-30 ° C for 1 hour, shake well to obtain a sample solution for later use.

[0030] Preparation of standard solution: take parthenolide reference substance and prepare standard solution with 0.2% and 0.1% content.

[0031] Detection: Using a 5ul quantitative capillary, take the same volume of sample solution and standard solution, respectively, and point them on TLC; use ethyl acetate to develop, after completion, dry the TLC plate and put it in iodine / silica gel for 5 minutes , remove the observation spot. Conclusion: by attaching figure 2 As a result, it can be seen that the sample spot and the reference substance spot have no spots at the same position, indicating that they do not contain the target compound. It can be judged that the extraction has been completed, and the extraction will...

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Abstract

The invention relates to a method for rapidly detecting parthenolide in plants and extracted residues. By comparing thin-layer chromatography spots of a sample solution and a standard solution, whether parthenolide is contained or not and the content range of parthenolide are judged. The device has the characteristics that the device is simple, the operation is easy, and the detection can be completed without professional equipment.

Description

technical field [0001] The patent belongs to the field of chemical substance identification and content determination, in particular to a rapid semi-quantitative detection method for the content of parthenolide. [0002] technical background [0003] Parthenolide belongs to the class of sesquiterpene lactones, which can be extracted from a variety of plants such as parthenolide or magnolia and was originally used to treat skin infections, rheumatism, and migraine. Recent studies have shown that parthenolide can inhibit the growth of prostate, breast, gastric, leukemia, kidney, lung, colon adenocarcinoma and other cancer cells. [0004] [0005] Several patents have described the preparation method of parthenolide. For example, in CN102241684B, the preparation method of parthenolide provided by the present invention solves the technical defects of low production yield of parthenolide and complicated technological process, and the preparation method of parthenolide provided...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/94G01N21/78
CPCG01N30/94G01N21/78
Inventor 李洪波邱传将
Owner ACCENDATECH LUOYANG CO LTD