Multi-stage flow channel blood vessel chip based on additive manufacturing

Abstract

The invention discloses a multi-stage flow channel blood vessel chip based on additive manufacturing in the field of bionic chips, the multi-stage flow channel blood vessel chip comprises a substrate and a double-layer blood vessel channel located on the surface of the substrate, the double-layer blood vessel channel comprises an inlet and an outlet which are used for circularly introducing intravenous endothelial cells, and a closure contraction part is arranged between the inlet and the outlet; the closure contraction part comprises elastic salient points which define a rectangular matrix, and the radius of the elastic salient points is larger than the distance between the elastic salient points. According to the technical scheme, a blood vessel chip structure is designed according to the characteristics of skull capillaries, so that in-vitro culture of the stent and the HUVECs is implemented, a blood vessel forming mechanism is analyzed, the cost of in-vitro culture of organs is reduced, an innovative design concept of the blood vessel embedded stent with the balustrade vein characteristics is provided, and the stent has a good application prospect. And in combination with in-vitro culture of dynamic perfusion, the vascularization process is disclosed better, and a new way is provided for regeneration of bone tissues.

Description

technical field [0001] The invention belongs to the field of bionic chips, in particular to a multi-stage flow channel blood vessel chip based on additive manufacturing. Background technique [0002] Microfluidic chip, also known as lab-on-a-chip, is a science and technology that forms a network with microchannels in a tiny volume and manipulates fluids at the micro and nano scales. Using this technology, basic operations such as sample preparation, reaction, separation, and detection; cell culture, sorting, and lysis involved in the fields of chemistry and biology can be realized. Due to the advantages of small size, flexibility, scale integration and low cost, it has been widely used in many fields such as physics, chemistry, electronic engineering, biology, medicine, nanoscience and so on. [0003] The vascular system is a closed network of arteries, veins, and capillaries. Blood circulation is a key element in the human body's gas exchange and large-scale nutrient deli...

AI Technical Summary

Problems solved by technology

The above two methods have their own advantages and disadvantages, but in the actual application process, the vascular network is constructed into a solid state, which cannot simulate the contraction process of blood vessels.

Images