Method for detecting feeding habits of lissorhoptrus oryzophilus in intestinal tract based on SCAR-PCR technology

A rice water weevil, OS-AGL-F technology, applied in recombinant DNA technology, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of heavy workload, unsuitable for research on quarantine insects, etc. High accuracy, reduced expertise requirements, good sensitivity results

Active Publication Date: 2022-07-08
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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Problems solved by technology

The observation method requires continuous observation to study the feeding of insects, and the workload is heavy, and the

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  • Method for detecting feeding habits of lissorhoptrus oryzophilus in intestinal tract based on SCAR-PCR technology
  • Method for detecting feeding habits of lissorhoptrus oryzophilus in intestinal tract based on SCAR-PCR technology
  • Method for detecting feeding habits of lissorhoptrus oryzophilus in intestinal tract based on SCAR-PCR technology

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Embodiment Construction

[0056] In order to make those skilled in the art understand the technical solutions of the present invention more clearly, the following examples are now given for illustration. It should be noted that the following examples do not limit the protection scope of the present invention.

[0057] The reagents, methods and equipment used in the following examples are all conventional reagents, methods and equipment in the technical field. The test methods that do not specify specific experimental conditions in the following examples are usually in accordance with conventional experimental conditions or in accordance with experimental conditions suggested by the manufacturer.

[0058] used in the following examples Plant Genomic DNA Kit was provided by Beijing Quanshijin Biotechnology Co., Ltd. Marine Animal Genomic DNA Kit was provided by Beijing Quanshijin Biotechnology Co., Ltd., 6×DNA Loading Buffer was provided by Beijing Qingke Biotechnology Co., Ltd., TS-Gelred Nucleic Ac...

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Abstract

The invention discloses a method for detecting feeding habits of lissorhoptrus oryzophilus in intestinal tracts of lissorhoptrus oryzophilus based on an SCAR-PCR technology, and relates to the technical field of molecular The method comprises the following steps: S1, extracting genome DNA (deoxyribonucleic acid) of a to-be-detected lissorhoptrus oryzophilus sample; s2, respectively carrying out SCAR-PCR (sequence characterized amplified region-polymerase chain reaction) reaction on the genome DNA by utilizing an SCAR primer pair LH-ycf4-F/R, OS-AGL-F/R and PD-ndhF-F/R; s3, performing a second round of SCAR-PCR reaction by taking the SCAR-PCR product in the step S2 as a template, wherein the primer is the same as that in the step S2; and S4, carrying out agarose gel electrophoresis on the SCAR-PCR product in the step S3, and analyzing an electrophoresis result. The method has the advantages of simple steps, short time consumption and high accuracy, can qualitatively evaluate the feeding habits of the lissorhoptrus oryzophilus, and greatly reduces the requirements on professional knowledge of workers.

Description

technical field [0001] The invention relates to the technical field of molecular biology detection, in particular to a method for detecting the food habit in the intestinal tract of rice water weevil based on SCAR-PCR technology. Background technique [0002] Agricultural pests mainly refer to herbivorous insects that damage crops by means of mouthpart feeding, mouthpart transmission, and egg laying, which reduces crop yield. Therefore, it is very important to study the feeding behavior of herbivorous insects, which not only helps to analyze the damage of herbivorous insects to crops and the community dynamics of herbivorous insects, but also can further study the interaction between herbivorous insects and crops. [0003] The feeding behavior of herbivorous insects is regulated by external and internal signals. The application of pesticides, the infestation of crops, and the parasitism of herbivorous insects by natural enemies will all affect the feeding behavior of herbivo...

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2565/125C12Q2531/113Y02A40/146
Inventor 袁龙宇雷浩霖肖汉祥李燕芳张振飞
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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