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Identification method of Yunnan small yellow ginger medicinal material

A technology of turmeric and turmeric gingerol, applied in the field of identification of turmeric herbs in Yunnan, to achieve the effects of strengthening systematization and standardization, high sensitivity, good repeatability and feasibility

Pending Publication Date: 2022-07-08
HENAN ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on Yunnan small yellow ginger focuses on planting, cultivation, nutritional components, etc., but there is no report on the identification of Yunnan small yellow ginger by NMR

Method used

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  • Identification method of Yunnan small yellow ginger medicinal material
  • Identification method of Yunnan small yellow ginger medicinal material
  • Identification method of Yunnan small yellow ginger medicinal material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Study on the identification of medicinal material A of Yunnan turmeric

[0036] 1) Supercritical extraction method to obtain characteristic extracts of gingerol and volatile oil

[0037] Weigh the medicinal materials or decoction pieces of Yunnan small turmeric and crush them, pass 20 mesh, and use the supercritical extraction method to extract the Yunnan small yellow turmeric. Turmeric curcumin and volatile oil characteristic extracts;

[0038] 2) Carbon NMR fingerprint detection of characteristic extracts of gingerol and volatile oil from Yunnan turmeric

[0039] Measure 100-120 mg of the above-mentioned Yunnan turmeric gingerol and the characteristic extract of volatile oil, dissolve in 0.5 mL of deuterated chloroform, and perform CNMR fingerprint detection to obtain Yunnan turmeric gingerol and volatile oil CNMR spectrum. Fingerprint.

[0040] 3) Carbon NMR fingerprint analysis of the characteristic extracts of gingerol and volatile oil from Yunnan turmeric

...

Embodiment 2

[0052] 1. Study on the identification of medicinal material B of Yunnan turmeric

[0053] 1) Supercritical extraction method to obtain characteristic extracts of gingerol and volatile oil

[0054] Weigh the medicinal materials or decoction pieces of Yunnan small yellow turmeric B and crush them, pass 20 mesh, and use the supercritical extraction method to extract the Yunnan small yellow ginger. The characteristic extracts of gingerol and volatile oil from Yunnan turmeric;

[0055] 2) Carbon NMR fingerprint detection of the characteristic extracts of gingerol and volatile oil from Yunnan turmeric

[0056] Measure 100-120 mg of Yunnan turmeric gingerol and the characteristic extract of volatile oil respectively, dissolve them in 0.5 mL of deuterated chloroform, and carry out carbon NMR fingerprint detection to obtain Yunnan turmeric gingerol and volatile oil carbon NMR fingerprints. Atlas.

[0057] 3) Carbon NMR fingerprint analysis of the characteristic extracts of gingerol an...

Embodiment 3

[0070] 1. Study on the identification of medicinal material E of turmeric in the market

[0071] 1) Supercritical extraction method to obtain characteristic extracts of gingerol and volatile oil

[0072] Weigh the commercially available turmeric E medicinal materials or decoction pieces and crush them, pass 20 meshes, and use the supercritical extraction method to extract the medicinal materials of Yunnan turmeric. Obtain the characteristic extracts of Yunnan turmeric gingerol and volatile oil;

[0073] 2) Carbon NMR fingerprint detection of the characteristic extracts of curcumin and volatile oil of the commercially available turmeric

[0074] Measure 100-120 mg of the commercially available turmeric E gingerol and volatile oil characteristic extract respectively, dissolve them in 0.5 mL of deuterated chloroform, and perform CNMR fingerprint detection to obtain Yunnan turmeric gingerol and volatile oil NMR carbon. Spectral fingerprint.

[0075] 3) Carbon NMR fingerprint an...

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Abstract

The invention belongs to the field of identification of natural medicinal plants, and discloses an identification method of Yunnan small yellow ginger medicinal materials. According to the method, the Yunnan small yellow ginger medicinal material is identified by adopting a nuclear magnetic resonance carbon spectrum fingerprint spectrum, the Yunnan small yellow ginger medicinal material is subjected to 6-gingerol quantitative analysis by adopting a high performance liquid chromatography in reference to a Chinese pharmacopoeia method, and the measured 6-gingerol content is 0.15%-0.20%; yunnan small yellow ginger varieties are not Yunnan small yellow ginger varieties beyond the range. The method can well reflect the category of the feature extracts in the Yunnan small yellow ginger medicinal material and the proportion of the feature extracts, and achieves the purpose of identifying the variety and quality of the Yunnan small yellow ginger medicinal material. The linear range is wide, the sensitivity is high, and the repeatability and feasibility are good. The method not only can effectively control the internal quality of the Yunnan small yellow ginger medicinal material, but also can objectively and effectively evaluate and control the quality of Yunnan small yellow ginger.

Description

technical field [0001] The invention belongs to the field of identification of natural medicinal plants, and in particular relates to a method for identifying the medicinal materials of Yunnan turmeric. Background technique [0002] Many areas in Yunnan belong to plateau basins, and the winter is colder. Yellow ginger is one of the necessary winter foods. As early as 1958, the small yellow ginger produced in Luoping area went abroad and became an important export commodity. The unique climate of Yunnan makes the nutritional content of Yunnan turmeric different from other places. Yunnan turmeric mainly contains oily volatiles such as gingerol, ginger oil terpenes, gingerene, phellandrene, citral, and aromatic oil, as well as gingerols, vitamins, gingerols, resins, starches, fibers and a small amount of minerals. substance. Has a good detoxification and sterilization and medicinal effects. At present, the research on Yunnan small yellow ginger focuses on planting, cultivati...

Claims

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Application Information

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IPC IPC(8): G01N24/08
CPCG01N24/087Y02P20/54
Inventor 张海艳赵天增陈飞常霞杨静廖怀玉韩红园陈欣
Owner HENAN ACAD OF SCI
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