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Quantum dot fluorescence immunochromatography test strip for detecting salmonella enteritidis and preparation method thereof

A Salmonella Enteritidis, fluorescence immunochromatography technology, applied in measurement devices, biological tests, analytical materials, etc., can solve the problems of poor stability, inability to quantitatively detect, low sensitivity, etc., achieve good stability, improve detection sensitivity, emission The effect of a narrow spectrum

Pending Publication Date: 2022-07-08
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Through the above analysis, the problems and defects of the existing technology are: the traditional colloidal gold immunochromatography technology relies on the aggregation of a large number of gold particles to achieve the effect visible to the naked eye, which makes the immunochromatographic detection products based on colloidal gold labels have low sensitivity and stability. Poor and not quantitatively detectable

Method used

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  • Quantum dot fluorescence immunochromatography test strip for detecting salmonella enteritidis and preparation method thereof
  • Quantum dot fluorescence immunochromatography test strip for detecting salmonella enteritidis and preparation method thereof
  • Quantum dot fluorescence immunochromatography test strip for detecting salmonella enteritidis and preparation method thereof

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preparation example Construction

[0050] like figure 1 As shown, the preparation method of the Salmonella Enteritidis quantum dot fluorescence immunochromatographic test strip provided in the embodiment of the present invention includes the following steps:

[0051]S101: Prepare quantum dot-labeled detection probe, take 25 μL of quantum dots, add 25 μL MES (several concentrations) and mix well; add 0.5 μL EDC (several mg / mL) and 0.5 μL NHS (several mg / mL), protect from light, and sonicate for 5 minutes Activated at 37°C for 30 min; centrifuged at 8000 g for 20 min and discarded the supernatant; resuspended in the pellet by adding 25 μL MES (several concentrations), then adding PBS (several concentrations and pH), and mixing; adding 10 μg Salmonella Enteritidis specific antibody and then adding PBS (several concentrations and pH), mix well, react at 37°C for 30 minutes in the dark; add blocking solution 100 mM glycine (containing 10 mg / mL BSA); 8000 rpm, RT, centrifuge for 15 minutes, discard the supernatant; r...

Embodiment 1

[0069] Example 1 (intended to use clinical samples to conduct an assessment of the actual performance of test strips)

[0070] 1. Materials

[0071] 1.1 Main reagents

[0072] Morpholineethanesulfonic acid (MES), 1-ethyl-(3-dimethylaminopropyl)carbodiimide (EDC), N-hydroxysuccinimide (NHS), dimethyl sulfoxide, Bovine serum albumin (BSA) was purchased from Sigma Company in the United States; Salmonella Enteritidis antibodies (Salmonella Enteritidis antibody labeling antibody and Salmonella Enteritidis capture antibody) were purchased from Zhongmei Xinxin Biotechnology Co., Ltd.

[0073] 1.2 Main instruments

[0074] Nitrocellulose membranes (NC membranes), glass cellulose membranes, sample pads, absorbent paper, and PVC bottom plates were all purchased from; film stripper, microcomputer automatic cutting machine, centrifuge, and electric heating blast drying oven.

[0075] 2. Method

[0076] S1, prepare quantum dot-labeled detection probe, take 25 μL of quantum dots, add 25...

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Abstract

The invention belongs to the technical field of salmonella enteritidis detection, and discloses a quantum dot fluorescence immunochromatography test strip for detecting salmonella enteritidis and a preparation method thereof. Assembling a quantum dot fluorescence immunochromatography test strip, coating a salmonella capture antibody Ab1 and goat anti-mouse IgG on an NC membrane by using a contact type automatic membrane scratching instrument to serve as a detection line T and a quality control line C, and drying; and assembling the sample pad, the combination pad, the NC membrane coated with the antibody and absorbent paper on a PVC (polyvinyl chloride) bottom plate, cutting into a test strip by using a numerical control strip cutting machine, and storing in a dark place under a dry condition for later use. The quantum dot fluorescence immunoassay test strip has the advantage of quantitative detection, and compared with a colloidal gold immunochromatography test strip which is mostly applied in the current market, the quantum dot fluorescence immunoassay test strip uses quantum dots as a marker, so that a fluorescence analyzer can be used for detecting a result, and the salmonella enteritidis can be quantitatively detected by detecting the fluorescence intensity of a detection line.

Description

technical field [0001] The invention belongs to the technical field of Salmonella Enteritidis detection, in particular to a fluorescent immunochromatographic test strip for detecting Salmonella Enteritidis quantum dots and a preparation method. Background technique [0002] At present, Salmonella is a common food-borne pathogen. It was first discovered in 1885, and was named Salmonella after Salmonella choleraesuis was isolated by Salmonella and others during the cholera epidemic. Salmonella now has 67 O-type antigens and more than 2000 serotypes, among which the Salmonella typhimurium serotype and the Salmonella enteritidis serotype dominate. Salmonella Enteritidis is a pathogenic bacteria that causes diarrhea in humans and animals, often causing intestinal diseases, manifested as fever, nausea, vomiting, abdominal pain, diarrhea. The number of diarrheal diseases is the largest, and Salmonella Enteritidis is the main pathogen. Therefore, the detection of Salmonella Enterit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/58G01N33/558G01N33/577G01N33/533
CPCG01N33/56916G01N33/588G01N33/558G01N33/582G01N33/533G01N33/577G01N2333/255G01N2469/10Y02A50/30
Inventor 陈海兰蒙淑玲刘添何家康于美玲张宁胡庭俊韦英益齐凯歌潘艳
Owner GUANGXI UNIV
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