Method for in-situ culture of bacterial cellulose hydrogel microspheres by using super-hydrophobic interface
A technology of bacterial cellulose and hydrogel microspheres, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as the inability to effectively prepare bacterial cellulose hydrogels, and it is easy to achieve the size of microspheres. , Simple operation, good quality of balls
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Embodiment 1
[0042] In order to elaborate on the principle of the method for in situ culturing bacterial cellulose hydrogel microspheres using superhydrophobic interfaces involved in the present invention, this embodiment will combine figure 1 Be explained. like figure 1 In (a), in this example, the exponentially proliferating Acetobacter xylinum medium is sprayed on the superhydrophobic interface 1 to form tiny medium droplets 3 . like figure 1 As shown in (b), in the subsequent culture process, tiny medium droplets 3 will be exposed to the air, and the spherical shape allows oxygen to enter the droplets uniformly from each surface of the droplets, which is Acetobacter xylinum 2 secretes bacterial cellulose 4 to provide the necessary material conditions. After a period of culture, such as figure 1 As shown in (c), the bacterial cellulose 4 secreted by Acetobacter xylinum 2 can directly form spherical bacterial cellulose hydrogel microspheres 5 .
Embodiment 2
[0044] In order to illustrate the process of the method for in situ culturing bacterial cellulose hydrogel microspheres using a superhydrophobic interface described in Example 1, this example will combine figure 2 Describe the specific preparation process involved in the present invention for in-situ culture of bacterial cellulose hydrogel microspheres using a superhydrophobic interface.
[0045] Step 1: Prepare a superhydrophobic interface 1, sterilize it by high temperature and high pressure, prepare a certain amount of exponentially proliferating Acetobacter xylinum medium and a sterile sprayer.
[0046] Step 2: Prepare a sterile environment.
[0047] Step 3: Put the exponentially proliferating Acetobacter xylinum medium into a sterile watering can, spray it over the superhydrophobic interface, and the mist droplets naturally fall on the superhydrophobic interface to form tiny medium droplets 3 .
[0048] Step 4: Incubate for 3 days at a suitable temperature in a sterile ...
Embodiment 3
[0051] In order to describe the bacterial cellulose hydrogel microspheres cultured in situ using the superhydrophobic interface described in Example 1, this example will combine image 3 Be explained. image 3 (a) in this example is a light microscope picture of the bacterial cellulose hydrogel microspheres 5. The prepared bacterial cellulose hydrogel microspheres 5 are translucent spherical and have good roundness. image 3 In (b) the field emission scanning electron microscope picture of bacterial cellulose hydrogel microspheres 5 in this example, the shape retention agent is filled in the microspheres, and the prepared bacterial cellulose hydrogel microspheres 5 have a diameter of At about 225um, the spherical shape is good and the surface is relatively flat. image 3 (c) in the middle is a field emission scanning electron microscope cross-sectional picture of bacterial cellulose hydrogel microspheres 5. The microspheres are not treated with a shape-retaining agent, and th...
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