Simple and efficient method for preparing salty peptide from wheat protein
A wheat protein and wheat peptide technology, applied in food science and other directions, can solve the problems of complex preparation process and many by-products of salty peptides, and achieve the effect of improving product flavor, easy absorption and prominent salty taste.
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Embodiment 1
[0033] A simple and efficient method for preparing wheat protein salty peptides, the steps are as follows:
[0034] (1) Proteolysis: prepare a 5% (w / v, g / ml, the same below) gluten aqueous solution, stir to form a uniform suspension, add flavored protease (Novozymes), and the ratio of protease to substrate is 3500U, 50 ℃ enzymatic hydrolysis 3h. After the enzymatic hydrolysis, the enzyme was boiled for 10 min to kill the enzyme, centrifuged at 3500 rpm for 15 min, and the supernatant was spray-dried to obtain wheat peptide powder.
[0035] (2) Heat treatment: make the peptide powder in step 1 into a 5% (w / v, the same below) aqueous solution, add 10% arginine by mass of the peptide powder, heat treatment at 110°C for 2 hours, and then drop to room temperature after the reaction is complete , the salty peptide was obtained after spray drying.
[0036] The salty taste evaluation of the peptide powder in step (1) and the salty peptide obtained in step (2) is as follows:
[0037...
Embodiment 2
[0044] A simple and efficient method for preparing wheat protein salty peptides, the steps are as follows:
[0045] (1) Proteolysis: prepare a 5% gluten aqueous solution, stir to form a uniform suspension, add flavored protease (Novozymes), and the ratio to the substrate is 3500U, and the enzyme is hydrolyzed at 50°C for 5h. After the enzymatic hydrolysis, the enzyme was boiled for 10 min to kill the enzyme, centrifuged at 3500 rpm for 15 min, and the supernatant was spray-dried to obtain wheat peptide powder.
[0046] (2) Heat treatment: compound the peptide powder in step 1 into a 5% aqueous solution, add 8% arginine by mass of the peptide powder, heat treatment at 90° C. for 4 h, after the reaction is completed, it is lowered to room temperature, and the salty peptide is obtained after spray drying.
[0047] The salty taste evaluation of peptide powder in step (1) and salty peptide in step (2) is as follows:
[0048] Salty evaluation Sensory evaluation electr...
Embodiment 3
[0051] A simple and efficient method for preparing wheat protein salty peptides, the steps are as follows:
[0052] (1) Proteolysis: prepare a 5% gluten aqueous solution, stir to form a uniform suspension, add flavored protease (Novozymes), and the ratio to the substrate is 3500U, and the enzyme is hydrolyzed at 50°C for 5h. After the enzymatic hydrolysis, the enzyme was boiled for 10 min to kill the enzyme, centrifuged at 3500 rpm for 15 min, and the supernatant was spray-dried to obtain the peptide powder.
[0053] (2) Heat treatment: The above peptide powder was prepared into a 5% aqueous solution, 10% arginine by mass of the peptide powder was added, heat treated at 110° C. for 2 hours, cooled to room temperature after the reaction, and spray-dried to obtain a salty peptide.
[0054] The salty taste evaluation of peptide powder in step (1) and salty peptide in step (2) is as follows:
[0055]
[0056]
[0057] In step (1), the degree of hydrolysis of gluten is 41.62...
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