Method for preparing high-purity rebaudioside b by alkaline hydrolysis of rebaudioside a

A high-purity and alkaline technology, applied in the field of stevioside, can solve the problems of high cost, low rebaudioside b content, and unsatisfactory yield, etc., and achieve the effects of high selectivity, high product purity, and high yield

Pending Publication Date: 2022-07-29
WENGYUAN GUANGYE QINGYI FOOD TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the content of rebaudioside b in the leaves of Stevia rebaudiana is very low, and obtaining it from nature is not only costly but also the output cannot meet the demand

Method used

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  • Method for preparing high-purity rebaudioside b by alkaline hydrolysis of rebaudioside a
  • Method for preparing high-purity rebaudioside b by alkaline hydrolysis of rebaudioside a
  • Method for preparing high-purity rebaudioside b by alkaline hydrolysis of rebaudioside a

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Weigh 50 g of rebaudioside a with a purity of 95% (mass percentage), add 1 L of water to dissolve, add saturated sodium hydroxide solution to adjust pH to 12, stir at a temperature of 95 ° C for constant temperature reaction for 4 h, add hydrochloric acid to adjust pH To 7.0, after standing at 20°C for 16 hours, a large amount of white precipitate was precipitated, which was washed with water after filtration (vacuum filtration), dried and detected by liquid phase.

[0032] The liquid chromatogram of rebaudioside a reference substance is as follows figure 2 As shown, the liquid chromatogram of the rebaudioside b reference substance is shown in image 3 As shown, the liquid chromatogram of the sample is shown in Figure 4 shown.

[0033] Qualitative and quantitative analysis of the reference substance and rebaudioside b sample of rebaudioside a and rebaudioside b by HPLC (the detection method refers to the national standard of steviol glycosides), the retention of the...

Embodiment 2

[0038] Weigh 50 g of rebaudioside a with a purity of 95%, add 1 L of water to dissolve, add saturated sodium hydroxide solution to adjust the pH to 12, stir at a temperature of 60 °C for constant temperature reaction for 4 h, add hydrochloric acid to adjust the pH to 7.0, and at 20 °C After standing for 16 hours, a large amount of white precipitates were precipitated. After filtration and washing, and liquid phase detection after drying, the yield of rebaudioside b was 76.3%, and the purity was 90.1%.

Embodiment 3

[0040] Weigh 50 g of rebaudioside a with a purity of 50%, add 1 L of water to dissolve, add saturated sodium hydroxide solution to adjust the pH to 12, stir and react at a constant temperature of 95 °C for 4 h, add hydrochloric acid to adjust the pH to 7.0, and adjust the pH to 7.0 at 4 °C. After standing for 16 hours, a large amount of white precipitates were precipitated. After filtration and washing, and liquid phase detection after drying, the yield of rebaudioside b was 83.3%, and the purity was 85.6%.

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Abstract

The invention discloses a method for preparing high-purity rebaudioside b by alkaline hydrolysis of rebaudioside a, which comprises the following steps: selecting rebaudioside a, adding water to obtain a rebaudioside a aqueous solution, adding alkali into the rebaudioside a aqueous solution to adjust the pH value to 9-12, reacting at a constant temperature of 60-100 DEG C for 2-24 hours, adding acid to neutralize after the alkaline hydrolysis reaction is completed, adjusting the pH value to 6-8, and filtering to obtain the high-purity rebaudioside b. And standing the neutralized reaction solution, separating out a crude product of rebaudioside b, and carrying out solid-liquid separation and drying on the crude product of rebaudioside b to obtain rebaudioside b. According to the method, glucosidic bonds of C19 in the rebaudioside a are selectively hydrolyzed without damaging glucosidic bonds of C13, the purpose of preparing the rebaudioside b from the rebaudioside a through an alkaline hydrolysis method is achieved, the converted rebaudioside b is easy to separate out, and subsequent separation and purification are simple and efficient.

Description

technical field [0001] The invention belongs to the technical field of stevioside, and in particular relates to a method for preparing high-purity rebaudioside b by alkaline hydrolysis of rebaudioside a. Background technique [0002] Stevia is a non-calorie natural sweetener extracted from the Asteraceae plant Stevia, and its sweetness is 200 to 450 times that of sucrose. The sweet component of stevia, steviol glycosides, has a common steviol skeleton, and each steviol glycoside has a unique taste and sweetness based on the number and arrangement of glucose units in its R1 and R2 positions (the structural formula of steviol glycosides is shown in figure 1 shown, where R1 is bit C19 and R2 is bit C13). Among the steviol glycosides, rebaudioside a has been widely used as a sweetener in food and beverages. However, its disadvantage is that it has a natural post-bitter or grassy taste, which also limits its further use as a sugar substitute. Rebaudioside b is also one of the ...

Claims

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Application Information

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IPC IPC(8): C07H15/256C07H1/00
CPCC07H15/256C07H1/00
Inventor 吕琪卫娜韩诗蕾廖金华
Owner WENGYUAN GUANGYE QINGYI FOOD TECH
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