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Trichoderma atroviride strain and application thereof in prevention and treatment of panax notoginseng root rot

A dark green Trichoderma, strain technology, applied in the application, fungicides, fungi and other directions, can solve the problems of chemical pesticide environmental pollution, drug resistance, pesticide residues, etc., to achieve the effect of reducing the use of

Active Publication Date: 2022-07-29
云南省农业科学院生物技术与种质资源研究所 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the serious occurrence of root rot of Panax notoginseng, and the problems of environmental pollution, pesticide residues, and drug resistance caused by chemical pesticide control, a strain of Trichoderma harzianum (Trichoderma harzianum) that can effectively control root rot of Panax notoginseng and its application

Method used

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  • Trichoderma atroviride strain and application thereof in prevention and treatment of panax notoginseng root rot
  • Trichoderma atroviride strain and application thereof in prevention and treatment of panax notoginseng root rot
  • Trichoderma atroviride strain and application thereof in prevention and treatment of panax notoginseng root rot

Examples

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Embodiment 1

[0028] A Trichoderma atroviride strain of the present invention, the Trichoderma dark green strain is PRWS21, the preservation name is Trichoderma atroviride, and it is preserved in the General Microorganism Center CGMCC of the China Microorganism Culture Collection Management Committee, and the address of the preservation unit is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, preservation date: April 6, 2021; preservation number: CGMCC No.21478.

[0029] The ITS gene sequence of the Trichoderma dark green strain is the nucleotide sequence shown in SEQ ID No.1.

[0030] The Trichoderma dark green inoculum prepared by the Trichoderma dark green strain of the present invention.

[0031] Its active ingredient is at least one of the following (a) (b) (c):

[0032] (a) the fermentation culture of Trichoderma dark green;

[0033] (b) the spore suspension of gained Trichoderma dark green;

[0034] (c) Sonication-lysed pellet of the resulting Trichoderma dark green c...

Embodiment 2

[0042] The difference between Example 2 and Example 1 is that the PDA liquid culture medium is composed of the following components: 200 g of potatoes, 5 g of glucose, set to 1 L of distilled water, pH 8, sterilized at 121° C. for 20 min. The PDA solid culture medium is composed of the following components: 200 g of potatoes, 10 g of glucose, 10 g of agar, set to 1 L of distilled water, pH 8, sterilized at 121° C. for 20 min.

Embodiment 3

[0044] The difference between Example 3 and Example 1 is that the PDA liquid culture medium is composed of the following components: 150 g of potato, 15 g of glucose, set to 1 L of distilled water, pH 7, sterilized at 121° C. for 20 min. The PDA solid culture medium is composed of the following components: 150 g of potatoes, 5 g of glucose, 12 g of agar, set to 1 L of distilled water, the natural pH is 7, and sterilized at 121° C. for 20 minutes.

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Abstract

The invention discloses a trichoderma atroviride strain and application of the trichoderma atroviride strain in prevention and treatment of panax notoginseng root rot. According to the trichoderma atroviride strain, the trichoderma atroviride strain is PRWS21, the preservation name is trichoderma atroviride, the trichoderma atroviride strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address of the preservation unit is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation date is April 6, 2021; the preservation number is CGMCC (China General Microbiological Culture Collection Center) The trichoderma atroviride is separated from rhizosphere soil of paris polyphylla, and has a good inhibition effect on panax notoginseng root rot fungi (fusarium oxysporum, fusarium solani and phytophthora cactorum) at the same time.

Description

technical field [0001] The invention relates to the technical field of microorganism application, in particular to a strain of Trichoderma dark green and its application in preventing and treating Panax notoginseng root rot. Background technique [0002] In recent years, with the sharp increase in the demand for traditional Chinese medicine resources, the wild resources of traditional Chinese medicine have been seriously damaged, and the conversion of wild to cultivated has become the only way to meet market demand. Wild Chinese medicinal materials rarely suffer from diseases, and even if they do, they will not cause disasters. After changing from wild to artificial cultivation, their growth environment has changed. Management measures such as seed adjustment have created conditions for diseases to occur and spread, and diseases are easy to spread within populations, between fields, and between regions, resulting in aggravation of diseases in cultivated medicinal materials. ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00A01N63/38A01P3/00C12R1/885
CPCC12N1/14C12N3/00A01N63/38
Inventor 郑宽瑜魏治镭张仲凯苏晓霞郑雪周凤陈永对吴阔
Owner 云南省农业科学院生物技术与种质资源研究所
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