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Method for culturing coral fungus mycelia

A culturing method and technology for coral fungus are applied in the field of culturing coral fungus mycelium, which can solve the problems of less research and achieve the effects of simple production, convenient operation and low production and processing cost.

Pending Publication Date: 2022-07-29
安康市农业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the research on coral fungi focuses on resource investigation, identification and classification, and chemical composition research, and there are few studies on how to improve the mycelium and polysaccharide content of coral fungi

Method used

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  • Method for culturing coral fungus mycelia
  • Method for culturing coral fungus mycelia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The culture method of the coral fungus mycelium of the present embodiment comprises the following steps:

[0017] S1, basal medium preparation: add 200g of potato, 10g of glucose and 10g of brown sugar to 1000ml of water and mix evenly, heat and boil for 20min, then filter and collect the filtrate; add 5g of red oyster mushroom powder and pine needle powder to the filtrate 4g, add water to make up to 1000ml, then divide into 250ml conical flasks, each bottle is divided into 100ml, seal the mouth of the conical flask with sealing film, and then sterilize at 121 ℃ for 30min, A fermentation medium is obtained; in the fermentation medium, the concentration of erythraea fungus powder is 5-10 g / L, and the concentration of pine needle fermentation liquid is 4-10 g / L;

[0018] The preparation method of the pine needle powder is to dry and pulverize the fresh pine needles in advance, and pass through an 80-mesh sieve;

[0019] S2, inoculate coral fungus bacterial classification...

Embodiment 2

[0023] The culture method of the coral fungus mycelium of the present embodiment comprises the following steps:

[0024] S1, basal medium preparation: add 200g of potato, 10g of glucose and 10g of brown sugar to 1000ml of water and mix evenly, heat and boil for 20min, then filter and collect the filtrate; add 10g of red oyster mushroom powder and pine needle powder to the filtrate 10g, add water to make up to 1000ml, then divide into 250ml conical flasks, each bottle is divided into 100ml, seal the mouth of the conical flask with a sealing film, and then sterilize at a temperature of 121 ℃ for 30min, obtain fermentation medium;

[0025] The preparation method of the pine needle powder is to dry and pulverize the fresh pine needles in advance, and pass through an 80-mesh sieve;

[0026] S2, inoculate the coral fungus strain on the fermentation medium obtained in S1 and ferment, the inoculum size of the coral fungus strain inoculation is 6%, and the fermentation temperature is ...

Embodiment 3

[0029] The culture method of the coral fungus mycelium of the present embodiment comprises the following steps:

[0030] S1, basal medium preparation: add 200g of potato, 10g of glucose and 10g of brown sugar to 1000ml and mix evenly, heat and boil for 20min, then filter and collect the filtrate; in the filtrate, add 7g of red oyster mushroom powder and 5g of pine needle powder , add water to make up to 1000ml, and then divide into 250ml conical flasks, each bottle is divided into 100ml, seal the mouth of the conical flask with a sealing film, and then sterilize at a temperature of 121 ℃ for 30min to obtain fermentation medium;

[0031] The preparation method of the pine needle powder is to dry and pulverize the fresh pine needles in advance, and pass through an 80-mesh sieve;

[0032] S2, inoculate the coral fungus strain on the fermentation medium obtained in S1 and ferment, the inoculation amount of the coral fungus strain inoculation is 6%, and the fermentation temperatur...

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Abstract

The invention provides a culture method of coral fungus mycelium, which comprises the following steps: adding 200g of potato, 10g of glucose and 10g of brown sugar into 1000ml of water, heating, boiling, filtering, and collecting filtrate; adding pleurotus djamor mushroom powder and pine needle powder into the filtrate, adding water to 1000ml, sub-packaging into conical flasks, sealing with a sealing film, and sterilizing to obtain a fermentation culture medium; and inoculating the fermentation culture medium with the coral fungus strain for fermentation to obtain the coral fungus mycelium. The yield of the obtained mycelium and exopolysaccharide is greatly increased, and the culture medium is easy to manufacture, convenient to operate, low in production and processing cost and capable of adapting to industrialized production and application.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, in particular to a method for culturing coral fungus mycelium. Background technique [0002] Coral fungus (Ramaria botrytoides), also known as broom fungus, broom fungus, named for its appearance like coral, belongs to Basidiomycetes, Polyporales, Coral fungus family (Clavariaceae), born on rot wood , mainly distributed in North China, Southwest my country and Northeast China, is one of the important wild edible mushroom resources in my country. "Southern Yunnan Materia Medica" records that coral fungus is sweet in taste, flat in nature, non-toxic, and treats stomach qi, expelling wind, breaking blood, and slowing down. Studies have shown that coral bacteria are rich in polysaccharides, proteins, amino acids and mineral elements, which have the functions of anti-oxidation and improving human immunity. [0003] At present, most of the research on coral fungus focuses on resource in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/14C12R1/645
CPCC12N1/38C12N1/14
Inventor 张园园李悦韩宝康王勇周高新丁建张晶
Owner 安康市农业科学研究院
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