Method for culturing coral fungus mycelia
A culturing method and technology for coral fungus are applied in the field of culturing coral fungus mycelium, which can solve the problems of less research and achieve the effects of simple production, convenient operation and low production and processing cost.
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Embodiment 1
[0016] The culture method of the coral fungus mycelium of the present embodiment comprises the following steps:
[0017] S1, basal medium preparation: add 200g of potato, 10g of glucose and 10g of brown sugar to 1000ml of water and mix evenly, heat and boil for 20min, then filter and collect the filtrate; add 5g of red oyster mushroom powder and pine needle powder to the filtrate 4g, add water to make up to 1000ml, then divide into 250ml conical flasks, each bottle is divided into 100ml, seal the mouth of the conical flask with sealing film, and then sterilize at 121 ℃ for 30min, A fermentation medium is obtained; in the fermentation medium, the concentration of erythraea fungus powder is 5-10 g / L, and the concentration of pine needle fermentation liquid is 4-10 g / L;
[0018] The preparation method of the pine needle powder is to dry and pulverize the fresh pine needles in advance, and pass through an 80-mesh sieve;
[0019] S2, inoculate coral fungus bacterial classification...
Embodiment 2
[0023] The culture method of the coral fungus mycelium of the present embodiment comprises the following steps:
[0024] S1, basal medium preparation: add 200g of potato, 10g of glucose and 10g of brown sugar to 1000ml of water and mix evenly, heat and boil for 20min, then filter and collect the filtrate; add 10g of red oyster mushroom powder and pine needle powder to the filtrate 10g, add water to make up to 1000ml, then divide into 250ml conical flasks, each bottle is divided into 100ml, seal the mouth of the conical flask with a sealing film, and then sterilize at a temperature of 121 ℃ for 30min, obtain fermentation medium;
[0025] The preparation method of the pine needle powder is to dry and pulverize the fresh pine needles in advance, and pass through an 80-mesh sieve;
[0026] S2, inoculate the coral fungus strain on the fermentation medium obtained in S1 and ferment, the inoculum size of the coral fungus strain inoculation is 6%, and the fermentation temperature is ...
Embodiment 3
[0029] The culture method of the coral fungus mycelium of the present embodiment comprises the following steps:
[0030] S1, basal medium preparation: add 200g of potato, 10g of glucose and 10g of brown sugar to 1000ml and mix evenly, heat and boil for 20min, then filter and collect the filtrate; in the filtrate, add 7g of red oyster mushroom powder and 5g of pine needle powder , add water to make up to 1000ml, and then divide into 250ml conical flasks, each bottle is divided into 100ml, seal the mouth of the conical flask with a sealing film, and then sterilize at a temperature of 121 ℃ for 30min to obtain fermentation medium;
[0031] The preparation method of the pine needle powder is to dry and pulverize the fresh pine needles in advance, and pass through an 80-mesh sieve;
[0032] S2, inoculate the coral fungus strain on the fermentation medium obtained in S1 and ferment, the inoculation amount of the coral fungus strain inoculation is 6%, and the fermentation temperatur...
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