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Lipopeptide-containing composition and application thereof in pest control

A pest control and composition technology, applied in the field of lipopeptide-containing compositions, can solve the problems of pesticide residue bio-resistance, etc., and achieve the effect of obvious insecticidal activity

Pending Publication Date: 2022-08-02
BIOLOGY INST OF HEBEI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Chemical control is currently a widely used measure, but problems such as pesticide residues and biological resistance caused by long-term use of chemical pesticides have become increasingly prominent

Method used

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  • Lipopeptide-containing composition and application thereof in pest control
  • Lipopeptide-containing composition and application thereof in pest control
  • Lipopeptide-containing composition and application thereof in pest control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Insecticidal effect of lipopeptide composition on Hemipteran pests

[0029]The three lipopeptides, iturin, fenggenin and surfactin, were prepared in sequence in a ratio of 1:1:1, the composition formed was dissolved in water, and a mother solution of 2000 μg / mL was prepared, and then the mother solution was diluted with water to 5 μg / mL, 10μg / mL, 20μg / mL, 200μg / mL, 500μg / mL, 1000μg / mL, 2000μg / mL liquid, treated with water as a control. The young stems of broad bean used in the insecticidal experiment of bean aphid (Aphiscraccivora Koch), the young cotton stems used in the insecticidal experiment of cotton aphid (Aphis gossypii), the young leaves of cabbage used in the insecticidal experiment of cabbage aphid (Brevicoryne brassicae), and the wheat long tube aphid ( For the young wheat stems used in the insecticidal experiment of Macrosipumavenae), take 20 mL of the above-mentioned chemical solutions of each concentration, soak the young plant stems and leaves ...

Embodiment 2

[0034] Example 2 Insecticidal effect of lipopeptide composition on coleopteran pests

[0035] The three lipopeptides, iturin, fenggenin and surfactin, were prepared in sequence in a ratio of 1:1:1, the composition formed was dissolved in water, and a mother solution of 2000 μg / mL was prepared, and then the mother solution was diluted with water to 5 μg / mL, 10μg / mL, 20μg / mL, 200μg / mL, 500μg / mL, 1000μg / mL, 2000μg / mL liquid medicine, with clear water as the control, according to the soil moisture to configure the medicinal soil, generally according to the liquid medicine and soil The mass-to-weight ratio is 1:1 to configure the medicinal soil. Use crushed peanuts as food, use a plastic bucket with a capacity of 4L as a container, put 2L of mixed medicinal soil in each bucket, and add an appropriate amount of food and mix well. Using the medicinal soil method, select the third instar larvae of the white star beetle, the dark gill beetle, the vermilion beetle and the small cloud ...

Embodiment 3

[0038] Example 3 Insecticidal effect of lipopeptide composition on Orthoptera pests

[0039] The three lipopeptides, iturin, fenggenin and surfactin, were prepared in sequence in a ratio of 1:1:1, the composition formed was dissolved in water, and a mother solution of 2000 μg / mL was prepared, and then the mother solution was diluted with water to 5 μg / mL, 10μg / mL, 20μg / mL, 200μg / mL, 500μg / mL, 1000μg / mL, 2000μg / mL liquid, treated with water as a control. The wheat seedlings used in the insecticidal experiment of locusts, the young peanut stems used in the insecticidal experiment of peanut crickets, the cotton crickets used in the insecticidal experiment of the mole cricket and the cotton katydid were soaked in the above-mentioned medicinal solution for 10 minutes, and the water was absorbed with filter paper. After placing it on a petri dish padded with filter paper, add 2 mL of deionized water for moisturizing, then cover the incubator with plastic wrap, poke holes, and add 1...

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Abstract

The invention discloses a lipopeptide-containing composition and application thereof in pest control, and belongs to the technical field of pest control. The composition comprises a lipopeptide in the abundant genin family, a lipopeptide in the iturin family and a lipopeptide in the surfactin family. By compounding the composition with different pesticides, the composition can be used for preventing and treating hemiptera pests, coleoptera pests, orthoptera pests, cockroach pests and lepidoptera pests, and particularly has very obvious insecticidal activity on hemiptera pests or coleoptera pests, and the insecticidal effect can reach 90% or above when the concentration is low for 24 hours, so that the composition can be used for preventing and treating the hemiptera pests or coleoptera pests. The composition disclosed by the invention can be applied to prevention and control of agricultural pests, and lays a foundation for development of new pest prevention and control drugs.

Description

technical field [0001] The present invention relates to the technical field of pest control, in particular to a composition containing a lipopeptide and its application in pest control. Background technique [0002] Fengyuansu, also known as fen camelina, includes fengycin A, B and plipastatin A, B. In 1986, German and Japanese teams simultaneously discovered fenggenin: fenggenin produced by Bacillus subtilis and phosphatidylcholine produced by Bacillus cereus. Fongenin was identified as an antifungal agent and phosphatidylserine was identified as an inhibitor of phospholipase A2. There are only minor structural differences between them (they differ in the conformation of amino acid tyrosine at position 9, L-form for phosphatidin and D-form for phosphatidylcholine). Then Kim et al. confirmed that Bacillus thuringiensis also produces fenggenin. Tosato et al. found an operon encoding fenggenin or phosphatidinostatin synthase in Bacillus subtilis 168, and then in Bacillus su...

Claims

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Application Information

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IPC IPC(8): A01N37/46A01N37/44A01N43/653A01N37/32A01N47/34A01N43/54A01N43/56A01N53/08A01N43/22A01N51/00A01N43/12A01N45/00A01N43/38A01N43/90A01N31/02A01N37/42A01N43/08A01N63/22A01N63/23A01N63/27A01N63/20A01N63/30A01N63/38A01N63/40A01P7/04A01C1/06
CPCA01N37/46A01N37/44A01N43/653A01N37/32A01N47/34A01N43/54A01N43/56A01N53/00A01N43/22A01N51/00A01N43/12A01N45/00A01N43/38A01N43/90A01N31/02A01N37/42A01N43/08A01N63/22A01N63/23A01N63/27A01N63/20A01N63/30A01N63/38A01N63/40A01C1/06Y02A50/30
Inventor 刘洪伟赵雯雅刘永锋姚彩苗张丽萍张飞燕王雅娜
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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