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Preparation of sophora flavescens alkaloid and sophora flavescens extract and application of sophora flavescens alkaloid and sophora flavescens extract in resisting inflammation and relieving itching

A technology of extracts and Sophora flavescens, which is applied in the fields of Sophora flavescens alkaloids, Sophora flavescens extract preparation, anti-inflammation and antipruritic applications, can solve the problems that Sophora flavescens alkaloids have not been reported, and reduce the number of scratches , the effect of inhibiting excessive secretion

Pending Publication Date: 2022-08-09
NOX BELLCOW COSMETICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Although many researches have been done on Sophora flavescens at home and abroad in recent years, there is no report on whether the alkaloids of Sophora flavescens have anti-inflammatory, soothing and antipruritic effects

Method used

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  • Preparation of sophora flavescens alkaloid and sophora flavescens extract and application of sophora flavescens alkaloid and sophora flavescens extract in resisting inflammation and relieving itching
  • Preparation of sophora flavescens alkaloid and sophora flavescens extract and application of sophora flavescens alkaloid and sophora flavescens extract in resisting inflammation and relieving itching
  • Preparation of sophora flavescens alkaloid and sophora flavescens extract and application of sophora flavescens alkaloid and sophora flavescens extract in resisting inflammation and relieving itching

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Example 1 Separation and preparation of active alkaloids of Sophora flavescens

[0072] The following implementation is a specific example, but the method of separation and preparation is not limited to this example.

[0073] 10 kg of dried stems of Sophora flavescens were crushed, extracted with ethanol (100 L, 30%) by leakage, and the extracts were combined under reduced pressure and concentrated to obtain 552 g of extract. The extract was dissolved in water to obtain a suspension, the pH was adjusted to 6 with HCl, and chloroform was added to separate the layers. Aqueous layer with 2% NH 3 ·H 2 Adjust the pH to 10 with O, and then extract with chloroform to obtain 221 g of total alkali extract.

[0074] 200g extract of total alkaloids of Sophora flavescens was separated by silica gel (1.5kg 800-100 mesh) column chromatography, and 5 fractions (Fr.1-5) were obtained by gradient elution with chloroform-methanol (100:50→50:100).

[0075] Based on the constructed DNC...

Embodiment 2

[0078] The separation and preparation of embodiment 2 Sophorae alkaloids

[0079] The following implementation is a specific example, but the method of separation and preparation is not limited to this example.

[0080]The 50% ethanol elution fraction with the best protective activity obtained in Example 1 was subjected to repeated silica gel chromatography column, chloroform-methanol gradient elution, and then separated and purified by ODS column chromatography and HPLC to obtain the compound shown in formula 1 (1.21 g), the compound represented by the formula 2 (1.01g), the compound represented by the formula 3 (989mg), the compound represented by the formula 4 (884mg), the compound represented by the formula 5 (921mg), the compound represented by the formula 6 (753mg), the compound represented by the formula Compound shown in 7 (669 mg).

[0081] Structural identification of Sophora flavescens alkaloid-1: Sophora flavescens alkaloid-1 is a light yellow oily substance, and ...

experiment example 3

[0131] Experimental Example 3: Sophora flavescens alkaloids, Sophora flavescens extract,

[0132] Cellular test of anti-inflammatory activity of the composition of Sophora alkaloids + Sophora Radix Sophorae Radix Extract

[0133] 5×10 3 Cells / well, NHDF cells (normal human skin fibroblasts) were seeded in a 96-well cell culture plate and cultured for 24h. The cells were treated with 2,4-dinitrochlorobenzene (10 μM) for 8 h. Cells were collected, washed twice with cold PBS, new medium was added, and a final concentration of 10 μg / mL Sophora alkaloids composition (Sophorae alkaloids-1: Sophorae alkaloids-2: Sophorae Radix Alkaloids-3) was added. : Sophora alkaloid-4: Sophora Radix Alkaloid-5: Sophora Radix Alkaloid-6: Sophora Radix Alkaloid-7 in a mass ratio of 1:1:1:1:1:1:1) or Sophora flavescens extract Or the composition of Sophora Radix alkaloid composition + Sophora flavescens extract (the mass ratio of Sophorae Radix alkaloid composition and Sophora flavescens extract i...

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Abstract

The invention discloses a method for preparing a sophora flavescens extract from dry sophora flavescens stems, a method for preparing sophora flavescens alkaloid compounds by an activity tracking method, and application of a composition of the sophora flavescens alkaloid compounds in anti-inflammatory and antipruritic cosmetics and drugs. The sophora flavescens alkaloid and the sophora flavescens alkaloid and sophora flavescens extract composition disclosed by the invention have excellent anti-inflammatory and itching-relieving effects, and the sophora flavescens alkaloid and the sophora flavescens extract show a remarkable synergistic effect, so that the sophora flavescens alkaloid and sophora flavescens extract composition have important significance in anti-inflammatory and itching-relieving aspects. The sophora flavescens alkaloid and sophora flavescens extract composition and the preparation thereof disclosed by the invention can be widely applied to anti-inflammatory and antipruritic cosmetics or medicines.

Description

technical field [0001] The present invention relates to a preparation method of Sophora flavescens alkaloid and Sophora flavescens extract and its application in anti-inflammatory and antipruritic cosmetics or medicines. Sophora flavescens extract and its use in anti-inflammatory, antipruritic cosmetics or pharmaceuticals. Background technique [0002] Pruritus is a skin pathological and physiological reaction caused by a variety of factors, without obvious primary damage. Western medicine mainly uses antihistamines to treat itchy skin. Although it can be cured, it has some side effects. Traditional Chinese medicine has a long history and rich experience in the treatment of skin diseases. It can control recurrent skin diseases or prolong the recurrence time, reduce or avoid the side effects of chemical drugs, delay the disease process and prolong the survival time. [0003] Sophora flavescens is the dried root of the legume Sophora Radix, with heat-clearing, antibacterial ...

Claims

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Application Information

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IPC IPC(8): C07D471/16C07D471/22C07D471/18A61P29/00A61P17/04A61K8/49A61Q19/00
CPCC07D471/16C07D471/22C07D471/18A61P29/00A61P17/04A61K8/4926A61Q19/00Y02A50/30
Inventor 范展华李于波郑冯锋顾葵
Owner NOX BELLCOW COSMETICS CO LTD
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