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Infrared finger print atlas identification technology for Chinese medicine

A fingerprint and infrared spectrum technology, which is applied in the field of traditional Chinese medicine infrared fingerprint identification technology, can solve the problems that the identification method has not been reported yet

Inactive Publication Date: 2004-09-01
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, fast, convenient, economical, accurate, and reliable identification methods have not been reported for the variety and authenticity identification of single medicinal materials or full prescriptions of Chinese patent medicines, especially when there are no morphological identification features.

Method used

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  • Infrared finger print atlas identification technology for Chinese medicine
  • Infrared finger print atlas identification technology for Chinese medicine
  • Infrared finger print atlas identification technology for Chinese medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1. To the identification of licorice root that can be crushed into powder solid sample

[0026] Crush the sample, pass through a 50-mesh sieve, weigh 2 g of the sample, add 30 ml of n-hexane, extract for 20 minutes, centrifuge, take the supernatant and evaporate to dryness to obtain the n-hexane extract; after the sample is evaporated to dryness, add 30 ml of ether, extract for 20 minutes minutes, centrifuge, take the supernatant and evaporate to dryness to obtain the ether extract; after the sample is evaporated to dryness, add 30ml of 95% ethanol, extract for 20 minutes, centrifuge, evaporate the supernatant to dryness, add 20ml of distilled water to the supernatant extract , to dissolve, centrifuge the suspension, add 30ml butanone to the supernatant, shake, centrifuge, take the supernatant and evaporate to dryness to obtain the butanone extract. Test the infrared spectra of the above three extracts respectively (see Figure 1-3 the first spectrum from th...

Embodiment 2

[0027] Embodiment 2. to the identification of honeyed pill sample Dahuoluodan

[0028] Weigh 3g sample, chop it up, add 30ml of distilled water, grind to make it a uniform suspension, shake, centrifuge, add 30ml of n-hexane to the supernatant, shake, centrifuge, take the supernatant and evaporate to dryness to get n-hexane alkane extract; add 30ml ether to the lower layer solution, shake, centrifuge, take the supernatant and evaporate to dryness to obtain the ether extract; add 30ml methyl ethyl ketone to the lower layer solution, shake, centrifuge, and evaporate the supernatant to dryness to obtain diethyl ether extract; Ketone extract. Test the infrared spectra of the above three extracts respectively (see Figure 4 ). Then compare its spectrum with the control or standard spectrum of Dahuoluodan and judge its authenticity according to its similarity.

Embodiment 3

[0029] Embodiment 3. Identification of granule sample Banlangen Granules

[0030] Take 1 g of the sample, add 20 ml of distilled water to dissolve, add 20 ml of ether, shake, centrifuge, evaporate the supernatant to dryness, and obtain the ether extract. Add 20ml butanone to the lower layer solution, shake, centrifuge, and evaporate the supernatant to dryness to obtain butanone extract. Test the infrared spectra of the above two extracts respectively (see Figure 12 ). Then compare its spectrum with the control or standard spectrum of Banlangen Granules and judge its authenticity according to their similarity.

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Abstract

The infrared finger print atlas identification technology is used in identification of natural plant material and Chinese medicine material, health article, beverage, seasoning, extraction and additive with plant material as main component. It includes the solvent extraction of sample, infrared spectrum test of the extraction, and comparison between the tested infrared spectrum and that of the reference. The solvent used in normal hexane and / or ethyl ether and / or butanone and the extraction is obtained through dissolution, extraction, and supernatant evaporation. The present invention can identify the sort of different medicine material and provide other information.

Description

Technical field: [0001] The invention relates to natural plants or traditional Chinese medicinal materials, Chinese patent medicines, health care products, beverages, seasonings, various extracts and additives with natural plants or natural plant raw materials as the main species identification and authenticity identification technology. Background technique: [0002] The infrared fingerprint identification technology of traditional Chinese medicine belongs to the infrared spectroscopy method in the instrumental analysis method. For other spectroscopic methods, such as: ultraviolet, fluorescence, atomic absorption, nuclear magnetic resonance, and chromatography-spectroscopy, some people have also used them to conduct research on the quality evaluation of traditional Chinese medicines. Various methods have different performances and their identification effects also have their own characteristics. Chromatography-spectroscopy can be used for accurate qualitative and quantitat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/25
Inventor 田进国
Owner SHANDONG UNIV
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