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Process for purifying swainsonine by biofermentation

A technology of swainsonine and biological fermentation, which is applied in the field of bioengineering research and can solve the problems of high cost and low content of swainsonine.

Inactive Publication Date: 2005-03-02
葛鹏斌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestically purified swainsonine is mainly derived from locoweed plants. Due to the low content of swainsonine in plants, there is still a gap in the main technical research of purification. If it is artificially synthesized, the cost will be very high

Method used

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  • Process for purifying swainsonine by biofermentation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: according to the technical scheme of the present invention, the process of purifying swainsonine is carried out as follows:

[0023] 1) Preservation of Rhizoctonia leguminosa 7-3: self-isolated Rhizoctonia leguminosa 7-3 PDA medium was inoculated, stored in a refrigerator at 4°C, and subcultured once every 20 days;

[0024] The formula of PDA medium consists of: peeled potato 200g, agar 15g, glucose 20g, water 1000ml;

[0025] 2) Inoculation, cultivation and collection of mycelium of Rhizoctonia leguminosa 7-3: In aseptic condition, inoculate strain 7-3 in Czapek's medium for biological fermentation, culture in aeration at 25°C for 2 weeks, and collect mycelia Body, dry, spare;

[0026] The formula of Czapek's medium consists of: 30g of sucrose, 3g of sodium nitrate, 1.3g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, 0.5g of potassium chloride, 0.01g of ferrous sulfate, 3g of yeast extract, and 1000ml of water.

[0027] 3) Purification of...

Embodiment 2

[0033] Embodiment 2: according to the technical scheme of the present invention, the process of purifying swainsonine is carried out in the following steps:

[0034] 1) Preservation of Rhizoctonia leguminosa 7-3: self-isolated Rhizoctonia leguminosa 7-3 PDA medium was inoculated, stored in a refrigerator at 4°C, and subcultured once every 25 days;

[0035] The formula of PDA medium consists of: peeled potato 1000g, agar 75g, glucose 100g, water 5000ml;

[0036] 2) Inoculation, cultivation and collection of mycelium of Rhizoctonia leguminosa 7-3: in aseptic condition, inoculate strain 7-3 in Czapek's medium for biological fermentation, culture in aeration at 27°C for 2 weeks, and collect mycelia Body, dry, spare;

[0037] The formula of Czapek's medium is as follows: 150g of sucrose, 15g of sodium nitrate, 6.5g of dipotassium hydrogen phosphate, 2.5g of magnesium sulfate, 2.5g of potassium chloride, 0.05g of ferrous sulfate, 15g of yeast extract, and 5000ml of water.

[0038]...

Embodiment 3

[0044] Embodiment 3: according to the technical scheme of the present invention, the purification process of swainsonine is carried out in the following steps:

[0045] 1) Preservation of Rhizoctonia leguminosa 7-3: self-isolated Rhizoctonia leguminosa 7-3 PDA medium was inoculated, stored in a refrigerator at 4°C, and subcultured once every 20 to 30 days;

[0046] The formula of PDA medium consists of: 2000g of peeled potatoes, 150g of agar, 200g of glucose, and 10000ml of water;

[0047]2) Inoculation, cultivation and collection of mycelia of Rhizoctonia leguminosa 7-3: In aseptic condition, inoculate strain 7-3 in Czapek's medium for bio-fermentation, culture in aeration at 26°C for 2 weeks, and collect mycelia Body, dry, spare;

[0048] The formula of Czapek's medium is as follows: 300g of sucrose, 30g of sodium nitrate, 13g of dipotassium hydrogen phosphate, 5g of magnesium sulfate, 5g of potassium chloride, 0.1g of ferrous sulfate, 30g of yeast extract, and 10000ml of w...

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Abstract

A biological fermenting process for purifying swainsonine includes such steps as biological fermenting engineering with pulse rhizoctonia 7-3 strain to culture its mycelia containing high content of swainsonine, extracting in alcohol solvent, cationic exchange, silica-gel column chromatography and segmental sublimation. Its advantages are low cost, high purity (more than or equal to 99%) and high output rate.

Description

1. Technical field [0001] The invention belongs to the field of bioengineering research, in particular to methods for biofermentation, biopharmaceuticals, natural product extraction, separation and identification, and in particular to a process for purifying swainsonine through biofermentation. 2. Background technology [0002] There are three sources of swainsonine: ① Extraction and separation from plants. At present, it is known that the plants containing swainsonin are mainly Astragalus and Oxytropis plants (locoweeds) of the leguminous family. These plants are widely distributed in All over the world, especially in North America and my country, the resources are very rich; followed by the leguminous horse bean plant, which is limited to Australia. ② Extract and isolate from Rhizoctonia leguminicola. Schneider et al. (1983) isolated swainsonine from Rhizoctonia leguminosa for the first time. ③ Synthetic swainsonine. In view of the many uses of swainsonine and its relate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/06
Inventor 童德文曹光荣赵宝玉葛鹏斌
Owner 葛鹏斌
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