Spectrum quantitative automatic analysis method

A quantitative analysis and automatic analysis technology, which is applied in the direction of material analysis, material analysis, and measuring devices through optical means, can solve the problems of limited number of attempts, inability to guarantee the quantitative analysis effect of quantitative parameters, time-consuming and labor-intensive problems, and achieve the goal of avoiding omissions , reduce analysis time and workload, and achieve accurate results

Inactive Publication Date: 2006-01-04
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

Although some existing practical experience can be used to help select quantitative parameters, for example, when using wavelength quantification, it is usually attempted to select a certain absorption peak wavelength of the sample for concentration measurement, which is still a time-consuming and laborious process
Moreover, due to the limitation of workload, the number of attempts is also limited, and it cannot be guaranteed that the determined quantitative parameters can always achieve the best quantitative analysis effect

Method used

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  • Spectrum quantitative automatic analysis method
  • Spectrum quantitative automatic analysis method
  • Spectrum quantitative automatic analysis method

Examples

Experimental program
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Embodiment

[0033] The invention is used in a spectrum analysis module of a small ultra-high-speed ultraviolet-visible spectrometer. Quantitative analysis was carried out on the spectral data of protein solutions with concentrations of 1.000mol / L, 0.500mol / L, 0.250mol / L and 0.125mol / L measured by the spectrometer. The spectra of the four concentrations of protein solutions are shown in the attached figure 2.

[0034] Using the method of the invention helps to select the quantitative wavelength when the spectrometer performs non-normalized wavelength quantification of the protein solution.

[0035] The implementation steps are as follows:

[0036] (1) Select the quantitative method. The spectral curve of the protein solution is shown in figure 2 . Because the spectrum of the protein solution has obvious absorption peaks and basically no background absorption, the non-normalized wavelength quantification method was chosen. At the same time, it is determined that there is only one qu...

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Abstract

The invention is an optical spectrum quantitative automatic analysis method, firstly determining an optical spectrum quantitative automatic analysis method of the four quantifying methods: non-normalization wavelength quantifying, normalization wavelength quantifying, area quantifying and speed quantifying and the quantitative parameters, then in the automatically ergodic circulating course, calculating light measurement the current values of the quantitative parameters, making linear fitting on the light measurement and measured quantity by using least squares method, recording all the quantitative parameters and corresponding related coefficients and finding the maximum related coefficient, and after the automatic ergodic course is finished, obtaining a 'related coefficient-quantitative parameter relation chart' and the suggested optimum quantitative parameter. It provides an automatic analysis method for the quantitative analysis result of optical spectrum, can evaluate quantitative measuring ability of the instrument for the special sample on given condition, gives the optimum quantitative analysis parameter and range and provides the reference for the users of spectral photometer and spectrograph.

Description

Technical field: [0001] The invention relates to an automatic analysis method for spectral quantification, which can be used to evaluate the quantitative measurement ability of an instrument for a specific sample under given conditions, automatically determine the optimal quantitative analysis parameters and their range, and achieve the best sample concentration measurement . It belongs to the technical field of spectrophotometry. Background technique: [0002] Quantitative analysis is one of the important components of spectrophotometric technology. One of the basic analysis contents is to calculate the concentration of the sample from the spectrum (absorbance) of the sample, and its theoretical basis is Beer's law. [0003] Many specific methods have been developed in practical work, such as wavelength quantification, normalized wavelength quantification, area quantification, velocity quantification, etc. Wavelength quantification is to select a wavelength point from th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/17
Inventor 倪一黄梅珍袁波林峰窦晓鸣
Owner SHANGHAI JIAO TONG UNIV
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