New liver tissue collagen diagnosis method
A technology of liver tissue and collagen, which is applied in ovulation diagnosis, medical science, fluorescence/phosphorescence, etc., can solve the problems of fluorescence intensity difference and unfavorable fluorescence observation of collagen tissue, so as to reduce manpower, facilitate early diagnosis and shorten diagnosis time Effect
Inactive Publication Date: 2006-06-14
北京地坛医院
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Problems solved by technology
It was found that under the excitation spectrum state of 415nm, there is a significant difference in the fluorescence intensity between the tumor tissue stroma and the benign lesion stroma
Excessively high excitation wavelength (510-590nm) is conducive to the observation of eosinophil particles, while the intrinsic fluorescence emission of tissue is deep red (>605nm), which is obviously not conducive to the observation of intrinsic fluorescence of collagen tissue
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[0029] The following describes the embodiments of the present invention with reference to the drawings.
[0030] The present invention is based on the existing reflection fluorescence microscope ( figure 1 ), use a hexagonal screwdriver to loosen the fixing screw on the right side of the vertical lighting device. Pull out the fluorescent assembly chamber 3. In the position of the fluorescent component ( figure 2 A) A model board 31 is installed. Loosen the fixing screws with a hexagonal screwdriver and remove the model board. Hold the fluorescence excitation block to be installed 4( figure 2 B) After aligning, push the dovetail into the seat frame in the direction of the arrow. Then tighten the fluorescent assembly fixing screws. Put the fluorescent assembly room back to its original position, and after pushing in, tighten the fixing screw on the right side of the vertical lighting device.
[0031] This diagnostic method follows the following steps:
[0032] 1. Install the spec...
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Abstract
The invention discloses a new method for diagnosing liver fibrosis. It uses liver puncture tissue specimens without any special chemical staining to directly excite the isolated liver tissue with collagen-specific excitation spectrum, and observes it with a fluorescent microscope system. The inherent fluorescence characteristics of liver tissue can be used to diagnose the degree of liver fibrosis. It is based on a fluorescent excitation block with a special excitation wavelength range of 300nm-460nm and a simple sample processing process, which can quickly, objectively and accurately diagnose the degree of liver fibrosis. Compared with the existing diagnostic equipment for liver fibrosis, the diagnosis of the degree of fibrosis has the advantages of significantly shortening the diagnosis time and reducing the consumption of manpower and material resources in the diagnosis process. It relates to the field of a special fluorescence excitation device for a fluorescence microscope used in the diagnosis of liver fibrosis and a corresponding sample processing method.
Description
Technical field [0001] The invention relates to a new liver fibrosis diagnosis equipment and method, in particular to a reflection fluorescence microscope special fluorescence excitation device used for liver fibrosis diagnosis and a corresponding liver fibrosis diagnosis method. Background technique [0002] Liver fibrosis is an inevitable pathological process of chronic liver disease. Hepatitis is an infectious disease with a high incidence worldwide, especially in East Asia and Southeast Asia. About 300 million people worldwide suffer from hepatitis. my country is an area with a high incidence of hepatitis. There are about 2 million cases of acute hepatitis each year, of which hepatitis A accounts for half (50%), hepatitis B accounts for 20-25%, hepatitis C accounts for 5-10%, and hepatitis E accounts for about Accounted for 10%. The total prevalence rate of hepatitis B virus in China is 59.17%, the infection rate is 57.6%, and the carrier rate is 9.8%. It is higher in rural a...
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IPC IPC(8): G01N21/64A61B10/00
Inventor 魏红山
Owner 北京地坛医院