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Oyster natural active peptide and its preparation method and its application in the preparation of anticancer medicine

A technology of active peptides and oysters, which can be used in drug combinations, pharmaceutical formulations, peptide/protein components, etc., and can solve the problems of lack of separation of oyster anti-tumor active substances

Inactive Publication Date: 2006-08-30
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The separation, purification and structural analysis of antimicrobial peptides, specific proteins and enzymes in oysters have been reported at home and abroad, but there is still a lack of separation and research on antitumor active substances in oysters

Method used

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  • Oyster natural active peptide and its preparation method and its application in the preparation of anticancer medicine
  • Oyster natural active peptide and its preparation method and its application in the preparation of anticancer medicine
  • Oyster natural active peptide and its preparation method and its application in the preparation of anticancer medicine

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Embodiment Construction

[0027] Oyster natural active peptide BPO-1 is obtained from fresh oysters after homogenization, centrifugation, acid extraction and Sephadex G-50 gel column chromatography, and is separated from the first elution peak. Group of natural active peptide components. see figure 1 , SDS-PAGE electrophoresis showed that the component was composed of 7 kinds of proteins with molecular weight between 29-97kD, respectively 29kD, 30kD, 32kD, 37kD, 57kD, 91kD and 97kD. exist figure 1 A is BPO-1, B is oyster acid crude extract, C is Maker, a is egg white lysozyme: 97000, b is trypsin inhibitor: 66200, c is bovine carbonic anhydrase: 43000, d is rabbit muscle Kinetin: 31000, e is bovine serum albumin: 20100, f is rabbit phosphorylase: 14400.

[0028] The separation and extraction method of BPO-1 is as follows (see figure 2 ):

[0029] 1. Extraction of BPO-1

[0030] The fresh and live monk's hat oysters were stored at a low temperature of 0-4°C. Take an appropriate amount, suspend i...

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Abstract

A natural active peptide is prepared from oyster through homogenizing, low-temp ultrahigh-speed centrifugal separation, freeze drying and chromatography. It is composed of 7 kinds of protein, whose molecular weights are 29KD, 30KD, 37KD, 57KD, 91KD and 97KD. It can be used to prepare the anticancer medicine as it can induce cancer cells to die, suppress reproduction and expression of cancer cells, and reducing the activity of enzyme associated with cancer.

Description

(1) Technical field [0001] The invention relates to a polypeptide isolated and extracted from nature for the first time, a preparation method and its application in the preparation of anticancer drugs. (2) Background technology [0002] Marine natural active substances are hotspots in modern biological research, and it has been found that they contain a variety of compounds with anti-tumor effects, and marine natural active peptides are an important part of the research on marine active substances. Oyster (Saccostrea cucullata) is a shellfish with high nutritional value and also a marine organism with great medicinal value. attention of some scholars. The separation, purification and structural analysis of antimicrobial peptides, specific proteins and enzymes in oysters have been reported at home and abroad, but there is still a lack of separation and research on the anti-tumor active substances in oysters. Yin Shumin etc. have reported the medicinal use of oysters (Chines...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/16A61P3/02
Inventor 李祺福
Owner XIAMEN UNIV