Sterilizing liquid for quick inactivating AIDS sex desease virus and sperm and its preparing method
A technology of AIDS virus and bactericidal solution, which is applied in the field of medicines and disinfection products, can solve problems in the experimental stage, achieve the effects of enhancing sensitivity and vitality, improving vaginal dryness, and promoting repair and healing
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Embodiment 1
[0039] Embodiment 1 prepares sterilizing liquid
[0040] (1) According to the formula description of each experimental scheme in Table 1, get the raw materials of corresponding weight respectively, mix them, make mixture:
[0041] raw material
[0042] (2) According to the formula of each experimental scheme in Table 2, take the raw materials of corresponding weight parts respectively, join in the mixture prepared in step (1), reconcile and stir into a transparent hydrogel:
[0043] raw material
[0044] (3) The filling machine divides into finished products.
Embodiment 2
[0045] The bactericidal effect experiment of embodiment 2 bactericidal liquid
[0046] 1. Experimental materials
[0047] Sample: the sterilizing solution prepared in Example 1, light yellow transparent liquid, stored at room temperature.
[0048] Test strains: Leptospira jaundice-hemorrhagic type 56601 and Leptospira typhoid type 56609 were used in the spirochete killing test; Neisseria gonorrhoeae 29106 was used in the gonorrhea killing test.
[0049] 2. Neutralizer test:
[0050] 2.1 Inspection basis: refer to Section 2.4 of the first volume of the "Technical Specifications for Disinfection" issued by the Ministry of Health.
[0051] 2.2 The neutralizer identification test adopts the suspension quantitative method, the action time of the bactericidal liquid and the test strain is 1 minute, and the neutralization time is 10 minutes. respectively at 37°C CO 2 Neisseria gonorrhoeae were cultured in an incubator and Leptospira were cultured in a 28°C incubator. The test wa...
Embodiment 3
[0066] Embodiment 3 sterilization liquid is to the killing effect experiment of HIV
[0067] 1. Test basis: "Virus inactivation test" in paragraph 2.1.1.10 of "Technical Specifications for Disinfection" (2002 edition).
[0068] 2. Experimental materials:
[0069] 2.1 Sample: the sterilizing solution prepared in Example 1, stored at room temperature.
[0070] 2.2 Human immunodeficiency virus: HIV~1 IIIB strain, passaged and cultured in MT4 cells.
[0071] 2.3 Cells: MT4 cells.
[0072] 2.4 Cell culture medium: RPMI 1640 culture medium containing 10 calf serum.
[0073] 2.5 Sterile deionized water.
[0074] 2.6 96-well cell culture plate.
[0075] 3. Experimental method:
[0076] Before the experiment, the sterilizing liquid samples were prepared into solutions of 1:10, 1:20, and 1:50 with sterile deionized water. Mix 0.9ml of samples with different concentrations (1:10, 1:20, 1:50) with 0.1ml of HIV-1 suspension, put them at room temperature for 1 minute, 5 minutes, and ...
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