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Method of utilizing quartz crystal microbalance in detecting virus in sample liquid

A technology of quartz crystal and microbalance, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of time-consuming verification process, insufficient sensitivity and specificity, and insufficient virus reliability, and achieves low detection cost and high detection efficiency. Fast results and easy detection

Inactive Publication Date: 2007-01-24
侯邦义
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With this method, the test result cannot be obtained until there is an obvious spectral effect, so it can be measured many days after the patient is ill, and the sensitivity and specificity are insufficient.
[0005] 3. Cell propagation and culture method: the virus isolated from the patient sample is propagated. This method can only provide evidence of active virus, and the verification process is time-consuming
[0009] However, Sauerbrey's law is only applicable to the situation where a solid hard quality is added to the quartz crystal in vacuum or in the air; for example, the Thickness Monitor (Thickness Monitor) used in vacuum evaporation monitors the metal and organic substances on the evaporation. The thickness or quality of the hard film is not suitable for the detection of test solutions, but virus detection is almost always the detection of test solutions such as blood, saliva, etc., so the reliability of this method for detecting viruses is not enough

Method used

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  • Method of utilizing quartz crystal microbalance in detecting virus in sample liquid
  • Method of utilizing quartz crystal microbalance in detecting virus in sample liquid
  • Method of utilizing quartz crystal microbalance in detecting virus in sample liquid

Examples

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Embodiment 1

[0025] Example 1 Detection of herpes virus (human herpes viruses)

[0026] Quartz crystal microbalance uses AT-cut circular quartz crystal chip, diameter 8.0mm, circular metal electrode diameter 4.0mm, thickness 200nm, plane fundamental wave, 10MHz quartz crystal probe, soaked in CMV65-kDe antibody solution first, so that the single The layer CMV65-kDe antibody adheres to the surface of the quartz crystal probe, and after drying, the original frequency f of the quartz crystal is measured o , then put the probe into a solution that has no virus but is the same as the solution to be tested, and measure the frequency value f of the quartz crystal under the condition of no virus in the solution 1 , according to Kanazawa's law, we know that the frequency value f 1 decrease, the frequency value f 1 with reference value f o The change value Δf between 1 =f 1 -f o .

[0027] The quartz crystal probe attached with CMV65-kDe antibody is immersed in the test solution that may cont...

Embodiment 2

[0029] Example 2 Detection of immunodeficiency virus (antihuman immunodeficiency viruses, HIV)

[0030] Quartz crystal microbalance uses AT-cut circular quartz crystal chip, diameter 8.0mm, circular metal electrode diameter 4.0mm, thickness 200nm (nanometer), plane fundamental wave, 10MHz quartz crystal probe, first soaked in anti-HIV manoclonel antibody solution In the process, the monolayer anti-HIV manoclonel antibody is adhered to the surface of the quartz crystal probe, and after drying, the original frequency f of the quartz crystal is measured o , and then put the probe into a solution that has no virus but is the same as the solution to be tested, and measure the frequency value f of the quartz crystal at this time 1 , according to Kanazawa's law, we know that the frequency value f 1 decrease, the frequency value f 1 with reference value f o The change value Δf between 1 =f 1 -f o .

[0031]Immerse the quartz crystal probe attached with anti-HIV manoclonel antib...

Embodiment 3

[0033] Embodiment 3 Simultaneous detection of different viruses

[0034] A quartz crystal microbalance application computer can array and use multiple quartz crystal probes at the same time. On different quartz crystal probes, the corresponding monoclonal antibodies of different viruses can be attached, and the frequency of each quartz crystal probe can be read at the same time. That is to say, a variety of corresponding trace viruses in the test solution can be detected at one time, and repeated cross-infection can also be detected.

[0035] In the above embodiments, the quartz crystal wafer used in the quartz crystal microbalance includes various cutting methods, such as AT, BT, CT, DT, FC, GT, SC and other cutting methods.

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Abstract

The method of utilizing quartz crystal microbalance in detecting virus in sample solution includes the following steps: adhering the electrode probe of the quartz crystal microbalance with antibody corresponding to the virus to be detected, drying and measuring the frequency f0 of the quartz in vacuum or air as the reference; setting the processed probe iniside solution without virus, measuring the frequency f1 and calculating the difference f1-f0; setting the processed probe inside the sample solution, measuring the frequency fm and calculating the difference fm-f0; and comparing the two differences to judge whether there is some virus in the sample solution or not based on whether the absolute value of fm-f0 is greater than that of f1-f0 or fm-f0=f1-f0. The said method is sensitive, low in test cost and fast and may be used widely in testing various kinds of virus.

Description

technical field [0001] The invention relates to a method for detecting viruses with a quartz crystal microbalance, in particular to a method for detecting viruses with a quartz crystal microbalance in a test solution. technical background [0002] In the prior art, there are the following methods for virus detection: [0003] 1. Molecular testing (PCR) method: that is, to directly test the viral nucleic acid genome sequence of the polymerase chain reaction. Because the genome sequence is often tens of thousands, the process of this method is time-consuming and costly. Similar viral genome sequences have more repetitions, resulting in poor detection sensitivity. [0004] 2. Immunofluorescence assay (Immunofluorescence assay): the antibody in the serum containing virus antibody is used as a test reagent and mixed with the serum of a suspected patient. If the patient's serum contains the virus, it will bind to the antibody of the test reagent, and Change the fluorescence spec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N5/00G01N33/569
Inventor 侯邦为
Owner 侯邦义