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Use of lysophosphatidylethanolamine (18:1) and lysophosphatidylinositol to retard senescence and to enhance fruit ripening

A technology of lysophospholipids and fruits, which is applied in the field of promoting fruit ripening and improving stability, and can solve problems such as the lack of research on the relative efficiency of lysophospholipids

Inactive Publication Date: 2001-05-30
WISCONSIN ALUMNI RES FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the relative efficiency of various LPEs and different types of lysophospholipids in controlling fruit ripening and enhancing its stability has not been studied.

Method used

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  • Use of lysophosphatidylethanolamine (18:1) and lysophosphatidylinositol to retard senescence and to enhance fruit ripening
  • Use of lysophosphatidylethanolamine (18:1) and lysophosphatidylinositol to retard senescence and to enhance fruit ripening
  • Use of lysophosphatidylethanolamine (18:1) and lysophosphatidylinositol to retard senescence and to enhance fruit ripening

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1：LPE(18∶1) and LPIPLD Embodiment 1

[0022] Examples of the present invention are now given, by way of illustration and not limitation. Example 1: Specific Inhibition of PLD by LPE (18:1) and LPI Example 1a: Chemicals and Plant Material

[0023]Natural lysophospholipids purified from egg yolk, beef liver and soybean as well as synthetic LPEs with different acyl chains (14:0, 16:0, 18:0, 18:1 ) were purchased from Avanti Polar Lipids (Alabaster, Alabama). The remaining phospholipid chemicals and materials used were purchased from Sigma (St. Louis, MO). Phospholipids and fatty acids were dissolved in chloroform:methanol:KOH (1N) (95:5:1, v / v). After adding water, the organic solvent was removed by flowing nitrogen. Before adding to the PLD reaction system, the concentration of the stock solution was adjusted to 1 mM with water. LPE solutions for the treatment of fruit and plant tissue were prepared in bulk by sonication of LPE powder suspended in water without the use of organic solvents.

[0024] Partially pur...

Embodiment 1b

[0026] Example 1b: Tissue sorting

[0027] Fully expanded leaves of two-month-old castor plants and cabbage were harvested, snap-frozen in liquid nitrogen, and homogenized with a mortar and pestle cooled on ice (13). An extraction buffer containing 50 mM Tris-HCL (pH 8.0), 10 mM KCl, 1 mM EDTA, 0.5 mM PMSF and 2 mM DTT was added to the powder sample. After grinding for an additional 5 minutes, the homogenate was centrifuged at 6000 g for 10 minutes to remove debris. The supernatant was centrifuged at 100,000 g for 30 minutes to separate the extract into soluble PLD and membrane-associated PLD. The resulting supernatant was collected as a soluble fraction, and the precipitate was collected as a membrane fraction. Wash the membrane fraction once with extraction buffer to remove soluble impurities. The soluble PLD and membrane-associated PLD samples were added to the reaction system to a final concentration of 100 μg / ml and 10 μg / ml, respectively. Embodiment 1c: PLD activity ...

Embodiment 1c

[0027] Fully expanded leaves of two-month-old castor plants and cabbage were harvested, snap-frozen in liquid nitrogen, and homogenized with a mortar and pestle cooled on ice (13). An extraction buffer containing 50 mM Tris-HCL (pH 8.0), 10 mM KCl, 1 mM EDTA, 0.5 mM PMSF and 2 mM DTT was added to the powder sample. After grinding for an additional 5 minutes, the homogenate was centrifuged at 6000 g for 10 minutes to remove debris. The supernatant was centrifuged at 100,000 g for 30 minutes to separate the extract into soluble PLD and membrane-associated PLD. The resulting supernatant was collected as a soluble fraction, and the precipitate was collected as a membrane fraction. Wash the membrane fraction once with extraction buffer to remove soluble impurities. The soluble PLD and membrane-associated PLD samples were added to the reaction system to a final concentration of 100 μg / ml and 10 μg / ml, respectively. Embodiment 1c: PLD activity detection method

[0028] The activi...

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Abstract

The present invention relates to a method of enhancing fruit ripening and stability and of delaying senescence in fruit and other plant tissues. This method consists of applying an effective amount of a lysophospholipid, such as lysophosphatidylethanolamine (18:1) (hereinafter referred to as LPE (18:1)) or lysophosphatidylinositol (hereinafter referred to as LPI) to the fruit and other plant tissues. Lysophospholipids such as LPE (18:1) and LPI were found to be superior to other lysophospholipids in delaying senescence and in inhibiting phospholipase D, a key enzyme in mediating membrane deterioration during of plant senescence. LPE (18:1) and LPI are naturally occurring and environmentally safe. Their use could replace many environmentally toxic compounds that are currently being used to retard senescence of flowers, fruits and leaves and to enhance fruit ripening.

Description

Background technique [0001] A variety of chemical and biological agents are currently used on commercially valuable fruit to control the timing of fruit ripening. The reagents can be used for many purposes. One purpose is to synchronize fruit ripening to facilitate efficient collection of fruit from the orchard. Another purpose is to prevent the fruit from falling to the ground so that the fruit remains on the branches until the appropriate ripening period. Another purpose of the fruit ripening agent is to increase the color development of the fruit so that the fruit has a better and more uniform color desired by the fruit retailer. It is common practice in the United States to treat many types of fruit during cultivation with one or more of these agents. [0002] Some agents previously used to control fruit ripening were purely synthetic agents that had the desired effect on the fruit of interest. Unfortunately, due to potential toxicity and carcinogenicity, some of these...

Claims

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Application Information

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IPC IPC(8): A23B7/153A01N57/12A23B7/154A23B7/16A23L3/3526A23L3/3553
CPCA23L3/3553A23B7/154A23L3/3526A23B7/16A01N57/12
Inventor 吉万·保罗·帕尔塔史蒂文·贝翁塔·留
Owner WISCONSIN ALUMNI RES FOUND
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