Gibberellic disease resistant and breeding method for wheat

A technology for wheat scab and scab, which is applied in the fields of botanical equipment and methods, plant genetic improvement, angiosperms/flowering plants, etc., which can solve the problems of difficulty in obtaining and a small number of cytological markers, and reduce the workload. , avoid blindness, reduce the effect of workload

Inactive Publication Date: 2007-02-14
JIANGSU ACAD OF AGRI SCI
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Problems solved by technology

The traditional identification of single-chromosomal substitution lines uses chromosome cytological markers, but the number of cytological markers is small and difficult to obtain. Therefore, before the emergence of molecular markers, single-chromosome substitution lines of wheat have not been identified.

Method used

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  • Gibberellic disease resistant and breeding method for wheat

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Embodiment Construction

[0029] 1. For the 6D chromosome monosomy of Chinese spring wheat, the number of chromosomes in the root tip meristematic cells was checked under a microscope, and the plants with 2n=41 were selected. The wheat head blight resistant cultivar Sumai 3 was inoculated to identify the resistance to head blight, and the single plant with the best resistance was selected, and the rate of diseased spikelets was 6.85%. The resistant single plant of Sumai 3 (♀) was used as the female parent, and crossed with Chinese spring wheat 6D chromosomal monosomy (♂) to obtain hybrid F1. The number of chromosomes in root meristematic cells of F1 plants was checked under a microscope, and the plants with 2n=41 and external morphology close to Chinese spring were selected as the male parent (♂), and then crossed with Chinese spring wheat 6D chromosomal monosomy (♀) to obtain BC1 plants. Repeat microscopic inspection, selection and hybridization until BC4 plants are obtained. The number of chromosome...

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Abstract

The invention belongs to the field of wheat aneuploid biotechnics breeding. Adopt the integral series of 'Chinese spring' wheat and the wheat gibberellic disease resistant source 'numble 3 su-wheat' to cross and backcross, and microscopy every generation, then select the integral to backcross again. Until it backcrosses to the forth generation, cover the separated integral generation in the bag to cross, and select homozygosity disome to be the substitute material of monosome. Select the SSR primer whose colorant layer is located to do PCR augmentation to the monosome, and certificate the monosome according to the gained molecular label. The material is good for positioning and colonying the gibberellic disease resistant genes of the numble 3 su-wheat, lead the resistant genes into the wheat breeds which don't contains that gene, seclect the wheat breed with gibberellic disease resistance, and accelerate the disease-resistant and the breeding process, then the selecting efficiency is improved.

Description

1. Technical field [0001] The invention belongs to the field of wheat aneuploid biotechnology breeding. 2. Background technology [0002] Wheat head blight caused by Fusarium graminearum (Schw) is a worldwide disease, which not only causes serious yield loss, but also deoxynivalenol (DON) and other mycotoxins seriously threaten the health of humans and animals. At present, the world's recognized sources of wheat scab resistance include China's Sumai 3, Ning 7840, Wangshuibai, etc., but these wheat varieties are difficult to be directly applied due to poor agronomic traits (such as plant height, yield, etc. in agricultural production. Therefore, it is necessary to determine the resistance genes carried by these resistance sources, and transfer them into conventional production varieties to breed disease-resistant varieties of wheat that can be used commercially. [0003] At present, many researchers (Kolb, F.L., Crop Sci, 2001, 41: 611-619; Buerstmayr, H., Theor Appl Genet...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H5/10
Inventor 张旭陆维忠姚金保周淼平任丽娟
Owner JIANGSU ACAD OF AGRI SCI
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