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Separation method for purifying gibberellin GA3

A technology for purification and separation of gibberellin, applied in the field of purification and separation of gibberellin GA3, which can solve the problems of affecting the growth regulation of GA3 plants, reducing the purity of GA3, and improving the purity of gibberellin, so as to save solvent consumption, Easy operation and high recovery rate

Active Publication Date: 2007-02-14
ZHEJIANG QIANJIANG BIOCHEMICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

GA 3 with GA 1 It is a pair of substances with similar polarities that exist in gibberellin at the same time. During the fermentation process, due to the increase of autolyzate, the change of pH, and the difference of strains, GA 1 The generation of GA, the traditional solvent extraction method and macroporous separation method cannot make GA 1 with GA 3 separate
From the perspective of physiological effects and application effects, gibberellin, as a plant growth regulator, is one of the GA 3 produced an effect, while GA 1 The presence of , then reduces the GA 3 The purity of , thus affecting the GA 3 plant growth regulation
Someone used ethanol, methanol, ethyl ester, acetone, etc. as solvents to treat gibberellin GA 3 Perform recrystallization in an attempt to remove GA 1 , but no significant effect, which makes gibberellin GA 3 The improvement of purity has become a major problem. Currently, gibberellin GA is registered in China 3 Only 85% pure

Method used

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  • Separation method for purifying gibberellin GA3

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Prepare 200ml of 50% acetone aqueous solution, heat up to boiling, slowly add 60g gibberellin original drug, gibberellin original drug GA 3 and GA 1 The contents are 93.4% and 3.5%, respectively. After adding, stir to make it dissolve into a transparent liquid, then quickly cool it to 25°C with a water bath, and when a large number of microcrystals are precipitated, carry out suction filtration, and wash the wet powder with 40ml of 50% acetone aqueous solution. After weighing, the wet powder was 71g, and then vacuum-dried to obtain 54.6g of crystal powder. detect GA 3 Content is 96.7%, GA 1 The content is 0.97%, the crystal powder recovery rate is 94.2%, and other indexes meet the indexes of the original medicine.

Embodiment 2

[0015] Prepare 200ml of 50% acetone aqueous solution, heat up to boiling, slowly add 60g gibberellin original drug, gibberellin original drug GA 3 and GA 1 The contents are 94.3% and 3.8%, respectively. After adding, stir to make it dissolve into a transparent liquid, then quickly cool it to 25°C with a water bath, and when a large number of microcrystals are precipitated, perform suction filtration, and wash the wet powder with 50ml of 50% acetone aqueous solution. After weighing, the wet powder was 70.5g, and then dried in vacuum to obtain 54.9g of crystal powder, which was detected by GA 3 Content is 97.2%, GA 1 The content is 0.89%, the crystal powder recovery rate is 94.3%, and other indicators such as water content and specific rotation are in line with the original drug indicators.

Embodiment 3

[0017] Prepare 200ml of 50% acetone aqueous solution, heat up to boiling, slowly add 60g gibberellin original drug, gibberellin original drug GA 3 and GA 1 The contents are 93.2% and 4.3%, respectively. After adding, stir to make it dissolve into a transparent liquid, then quickly cool it to 25°C with a water bath, and when a large number of microcrystals are precipitated, perform suction filtration, and wash the wet powder with 45ml of 50% acetone aqueous solution. After weighing, the wet powder was 69.7g, and then dried in vacuum to obtain 55.2g of crystal powder, which was detected by GA 3 Content is 96.4%, GA 1 The content is 1.08%, the crystal powder recovery rate is 95.1%, and other indicators such as water content and specific rotation are in line with the original drug indicators.

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Abstract

The present invention relates to a purification and separation method of gibberellin GA3. The gibberellin can be used as plant growth regulating agent, in which G3 is its effective component. Said invention is characterized by using boiling 50% acetone as solvent to make recrystallization so as to greatly raise purity of gibberellin GA3.

Description

technical field [0001] The present invention relates to a kind of purification method of plant growth regulator, particularly relate to a kind of gibberellin GA 3 purification and separation methods. Background technique [0002] Gibberellin is a plant growth regulator widely used internationally and domestically, and it plays a regulating role in preserving flowers and fruits of fruits, rice and other plants. GA 3 with GA 1 It is a pair of substances with similar polarities that exist in gibberellin at the same time. During the fermentation process, due to the increase of autolyzate, the change of pH, and the difference of strains, GA 1 The generation of GA, the traditional solvent extraction method and macroporous separation method cannot make GA 1 with GA 3 separate. From the perspective of physiological effects and application effects, gibberellin, as a plant growth regulator, is one of the GA 3 produced an effect, while GA 1 The presence...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D307/93
Inventor 裘国寅姚萍华
Owner ZHEJIANG QIANJIANG BIOCHEMICAL CO LTD