Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Group culture medium of Spotleaf-orchis and rapid breeding method thereof

A technique for tissue culture of orchids, which is applied to the optimized medium formula of orchids tissue culture seedlings and the field of rapid propagation, can solve problems such as no successful tissue culture, and save rare and endangered plants, Good applicability and the effect of reducing production costs

Inactive Publication Date: 2007-06-06
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although variegated orchid belongs to the orchid family, there is no report of successful tissue culture so far; variegated orchid has certain requirements for light, temperature, water, fertilizer, and gas from seed structure to plant growth and development, so no matter the group Cultivating seedlings or transplanting tissue cultured seedlings, how to explore and create conditions to meet the specific requirements of variegated orchid plants for the above factors is the key to the success of tissue culture

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 (the medium 1 that is suitable for the tissue culture of variegated leaf orchid)

[0030] 1) Basic medium: select MS medium, in which sucrose is 20, agar is 9g / L, pH5.0;

[0031] 2) The induction medium is MS+6-BA 2.0mg / L+NAA 0.2mg / L;

[0032] 3) The proliferation medium is MS+6-BA 5.0mg / L+IBA 0.1mg / L;

[0033] 4) Strong seedling medium is MS+6-BA 2.0mg / L+NAA 0.5mg / L;

[0034] 5) The rooting medium is MS+IBA 0.1mg / L+NAA 0.1mg / L+activated carbon 1g / L;

Embodiment 2

[0035] Embodiment 2 (the substratum 2 that is suitable for variegated leaf orchid tissue culture)

[0036] 1) The basic medium is the improved MS medium (1 / 2MS), the sugar is 30g / L, the agar is 8g / L, and the pH is 6.0;

[0037] 2) The induction medium is 1 / 2MS+6-BA 5.0mg / L+IBA 0.4mg / L;

[0038] 3) The proliferation medium is 1 / 2MS+6-BA 2.0mg / L+NAA 0.3mg / L;

[0039] 4) Strong seedling medium is 1 / 2MS+6-BA 0.5mg / L+IBA 0.3mg / L;

[0040] 5) The rooting medium is 1 / 2MS+IBA 0.2mg / L+NAA 0.3mg / L;

Embodiment 3

[0041] Embodiment 3 (optimization medium suitable for the tissue culture of the variegated leaf orchid)

[0042] 1) Basic medium: choose MS or 1 / 2MS medium, in which sucrose is 30g / L, agar is 7g / L, pH5.4;

[0043] 2) The induction medium is MS+6-BA 3.0mg / L+IBA 0.5mg / L;

[0044] 3) The proliferation medium is MS+6-BA 2.0mg / L+NAA 0.5mg / L;

[0045] 4) Strong seedling medium is MS+6-BA 1.0mg / L+NAA 0.1mg / L;

[0046] 5) The rooting medium is 1 / 2MS+NAA 0.5mg / L+IBA 0.5mg / L+activated carbon 1g / L;

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses the group culture medium of Spotleaf-orchis and rapid breeding method, the Spotleaf-orchis in the nature generally employs asexual propagation, but the rate of reprothropology is very low. The invention realizes strong targeting performance, good application effect, and high seedling inductivity of over 90%.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to an optimized medium formula and a rapid propagation method of the orchid plant Orchid variegata tissue culture seedlings. Background technique [0002] Variegated leaf orchid (Goodyera schlechtendaliana), also known as Xiaoyeqing, belongs to Orchidaceae. It is distributed in Jiangsu, Zhejiang, Hubei, Guizhou, Sichuan, Guangdong, Fujian and other provinces in China. It is mainly produced in Dapan Mountain and Tianmu Mountain. It is a rare and endangered medicinal product. Plant resources, cold in nature, mild in taste; clearing lung and relieving cough, treating bronchitis, detoxifying and reducing swelling, promoting blood circulation and relieving pain, softening and resolving hard masses; externally used to treat poisonous snake bites, carbuncle boils and sores; in addition, the leaves of variegated orchid are very beautiful and can be used as ornamental Plant cult...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
Inventor 徐刚汪一婷牟豪杰吕永平陈剑平陈炯
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products