Group culture medium of Spotleaf-orchis and rapid breeding method thereof
A technique for tissue culture of orchids, which is applied to the optimized medium formula of orchids tissue culture seedlings and the field of rapid propagation, can solve problems such as no successful tissue culture, and save rare and endangered plants, Good applicability and the effect of reducing production costs
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Embodiment 1
[0029] Embodiment 1 (the medium 1 that is suitable for the tissue culture of variegated leaf orchid)
[0030] 1) Basic medium: select MS medium, in which sucrose is 20, agar is 9g / L, pH5.0;
[0031] 2) The induction medium is MS+6-BA 2.0mg / L+NAA 0.2mg / L;
[0032] 3) The proliferation medium is MS+6-BA 5.0mg / L+IBA 0.1mg / L;
[0033] 4) Strong seedling medium is MS+6-BA 2.0mg / L+NAA 0.5mg / L;
[0034] 5) The rooting medium is MS+IBA 0.1mg / L+NAA 0.1mg / L+activated carbon 1g / L;
Embodiment 2
[0035] Embodiment 2 (the substratum 2 that is suitable for variegated leaf orchid tissue culture)
[0036] 1) The basic medium is the improved MS medium (1 / 2MS), the sugar is 30g / L, the agar is 8g / L, and the pH is 6.0;
[0037] 2) The induction medium is 1 / 2MS+6-BA 5.0mg / L+IBA 0.4mg / L;
[0038] 3) The proliferation medium is 1 / 2MS+6-BA 2.0mg / L+NAA 0.3mg / L;
[0039] 4) Strong seedling medium is 1 / 2MS+6-BA 0.5mg / L+IBA 0.3mg / L;
[0040] 5) The rooting medium is 1 / 2MS+IBA 0.2mg / L+NAA 0.3mg / L;
Embodiment 3
[0041] Embodiment 3 (optimization medium suitable for the tissue culture of the variegated leaf orchid)
[0042] 1) Basic medium: choose MS or 1 / 2MS medium, in which sucrose is 30g / L, agar is 7g / L, pH5.4;
[0043] 2) The induction medium is MS+6-BA 3.0mg / L+IBA 0.5mg / L;
[0044] 3) The proliferation medium is MS+6-BA 2.0mg / L+NAA 0.5mg / L;
[0045] 4) Strong seedling medium is MS+6-BA 1.0mg / L+NAA 0.1mg / L;
[0046] 5) The rooting medium is 1 / 2MS+NAA 0.5mg / L+IBA 0.5mg / L+activated carbon 1g / L;
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