High-molecule porous scaffold for tissue engineering and its preparing process by hot pressing

A porous scaffold and tissue engineering technology, applied in the fields of biomedical engineering and polymer materials, can solve the problems of low pore density, difficult solvent volatilization and demoulding, unfavorable transport of cell nutrients and metabolites, etc. The effect of good shape retention

Inactive Publication Date: 2002-10-30
FUDAN UNIV
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Problems solved by technology

[0005] In the solvent casting method, the existence of a large amount of solvent makes the polymer / solvent / porogen composite have good fluidity, so it can be cast and formed to prepare porous scaffolds with simple shapes, but it is difficult to prepare porous scaffolds with complex shapes and a thickness of more than 2mm. For porous scaffolds, it is very difficult to volatilize the solvent and release the mold, so there is no report in the literature on the preparation of scaffolds with complex shapes by solvent casting
On the other hand, the solvent-casting method often leads to the formation of a surface cortex when preparing porous scaffolds, which is detrimental to the engraftment or ingrowth of cells and the transport of nutrients and metabolites in tissue engineering applications.
As an improvement to the solvent casting / particle leaching method, the inventors proposed a new method for preparing degradable polymer porous scaffolds for tissue engineering—solvent-based cold pressing / particle leaching method. The porous scaffold prepared by this method not only It has a connected porous structure, and the surface of the scaffold is also porous, which avoids the formation of the surface skin layer, but the pore density on the surface of the scaffold is lower than that of the bulk

Method used

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  • High-molecule porous scaffold for tissue engineering and its preparing process by hot pressing
  • High-molecule porous scaffold for tissue engineering and its preparing process by hot pressing

Examples

Experimental program
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Effect test

Embodiment 1

[0048] Example 1 First, make a human ear model with gypsum, put it into a copper mold box, and pad the bottom flat; mix the silicone rubber main agent and curing agent in a ratio of 10 grams: 0.68 grams, then fill it into the mold box, and compact it , cured at room temperature for 5 minutes, demolded to obtain a flexible mold A (female mold A); put the female mold A into the mold box, and then put the human ear model, repeat the above process to obtain the female mold B; at the same time, design and process Copper rigid mould, so that flexible mold can just be put into rigid mould; 0.3 gram of viscosity-average molecular weight is 55000 PCL is dissolved in the chloroform solution that forms PCL in 6 gram of chloroforms, the sodium chloride porogen particle is sieved, take Add 2.7 grams of sodium chloride particles with a particle size of 280-450 μm into the solution (the amount of sodium chloride accounts for 90% of the total weight of polymer PCL and sodium chloride), stir ev...

Embodiment 2

[0049] Example 2 The amount of PCL used is 0.15 g, the amount of chloroform used is 6 g, the amount of sodium chloride particles is 2.85 g, and the others are the same as in Example 1. A PCL porous scaffold with a complex shape of the human ear was obtained with a porosity of 92.9%, highly connected pores, and a high pore density on the scaffold surface.

Embodiment 3

[0050]Example 3 The polymer used is PDLLA with a viscosity-average molecular weight of 41,000, the dosage is 0.45 g, the dosage of chloroform is 6 grams, the dosage of sodium chloride particles is 2.55 grams, and the hot pressing temperature is 120°C. Others are with embodiment 1. A PDLLA porous scaffold with a complex shape of the human ear was obtained with a porosity of 78.9%, highly interconnected pores, and a high pore density on the scaffold surface.

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Abstract

A high-molecular porous scaffold for tissue engineering is prepared from biodegradable and biocompatible high-molecular material through designing flexibility-rigidity combined mould and hot press/particle infusion. Said process scaffold has complex shape similar to the damage tissue or organ and communicated porous structure. Its porosity is more than 90%.

Description

technical field [0001] The invention belongs to the technical field of polymer materials and the field of biomedical engineering, and specifically relates to a biodegradable polymer porous scaffold and a preparation method thereof, which is an extracellular matrix substitute for tissue engineering with the same complex shape as a defective tissue or organ. technical background [0002] Tissue engineering, as a new method of using living cells to reconstruct or repair tissues or organs, overcomes the problems of donor shortage and immune rejection in traditional tissue or organ transplantation, making the treatment of tissue or organ defects start from organ transplantation. Enter a new era of organ manufacturing. The basic principle and method of tissue engineering is to plant human cells cultured in vitro on tissue engineering porous scaffolds that have good biocompatibility and can be gradually degraded and absorbed in vivo to form cell-scaffold complexes. Cells proliferat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/02A61L27/50
Inventor 丁建东吴林波
Owner FUDAN UNIV
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