Chemical weedicide induced promoter and application thereof

Abstract

The present invention belongs to the field of genje engineering technology. It particular, it relates to a promoter segment capable of separating and utilizing nucleic acid. Said promoter is originated from some genes which are sensitive to sulfonylurea compounds (such as chlor-sulfuron and ethametsulfuron) and related compounds in the plants of rice, etc. The nucleic acid sequence of the coded desired target product gene and one above-mentioned promotor segment are fused to obtain recombinant DNA, then this recombinant DNA is used to transform plant, and a new transgenic plant can be obtained. The expression of target gene being in said recombinant DNA in the new plant is controlled by proper chemical inducer.

Description

technical field

[0001] The invention belongs to the technical field of genetic engineering, and specifically relates to a promoter fragment for isolating and utilizing nucleic acid. The promoter is derived from p-sulfonylurea compounds (such as chlorsulfuron, ethamuron) and related Some genes to which the compound is sensitive. The nucleic acid sequence encoding the desired target product gene and one of the above-mentioned promoter fragments are fused to obtain a recombinant DNA, and the plant is transformed with the recombinant DNA to obtain a new transgenic plant. In new plants the expression of the gene of interest in the recombinant DNA is regulated by suitable chemical inducers. Background technique

[0002] Transgenic technology has become a powerful tool to solve the problems encountered by multicellular organisms, and it is also true in the field of plants, which includes introducing endogenous or exogenous genes into plants to modify certain functions or change ge...