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Specimen having capability of separating solid component

A test piece and solid technology, applied in the field of chemical analysis of biological materials, can solve the problems of deviation, difficulty and complexity of the concentration distribution of the tested substance

Inactive Publication Date: 2003-07-09
ARKRAY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since blood cells tend to diffuse on the reagent layer like the above-mentioned multi-layer test piece, there are still problems in the measurement of transmitted light, such as a decrease in oxygen permeability associated with the formation of the blood cell layer, and the sensitivity of the blood cell layer to light. block
Also, in these test pieces, when blood plasma spreads in layers, dyes and other substances produced by reacting with coloring reagents also spread further, causing deviations in the concentration distribution of test substances
[0011] Also, because the test pieces are produced by immobilizing the polymer particles with a binder, which creates the proper voids between the particles, their production is complicated and difficult

Method used

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  • Specimen having capability of separating solid component
  • Specimen having capability of separating solid component
  • Specimen having capability of separating solid component

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] First, the bottom layer will be described. In Examples of the present invention, Milli-Q water obtained by treating distilled water in Milli-Q Labo (manufactured by Nippon Millipore Co., Ltd.) was used. The distilled water was produced by using a pure water producing apparatus Autostill WG 220 type (manufactured by Yamato Scientific Co., Ltd.).

[0093] First, after cleaning the transparent polystyrene plate with ethanol-Milli-Q water and drying it, it was irradiated with ultraviolet rays for 1 - 30 minutes (preferably 3 minutes), and then make the surface of the above-mentioned transparent polystyrene plate undergo hydrophilic treatment to make the bottom layer 1 . The shape of the hydrophilic region of the bottom layer 1 was made into a gourd shape formed by joining two circles with a diameter of 5 mm.

[0094] Next, the manufacture of the reagent layer will be described.

[0095] Inorganic gelling agent Laponite XLG, Bis-Tris buffer solution (pH6.5), POD (peroxida...

Embodiment 2

[0105] Next, except that the amount of raponite XLG and other reagents was fixed, and the amount of particles used was changed according to the following Table 2, test pieces with different reagent layer thicknesses were manufactured in the same manner as in Example 1, and the expected reagent layer thickness was measured . The expected thickness is the film thickness (μm) obtained by observing the cross-section of the reagent layer on the SEM and calculating it.

[0106] Whole blood was spotted on each test piece with different reagent layer thicknesses, and the migration distance of plasma was measured. The migration distance is the migration distance (mm) of the separated plasma from the spotting position to the top within 5 seconds of spotting the whole blood.

[0107] Reagent formula

[0108] As can be seen from Table 2, the thickness of the reagent layer varies according to the amount of particles used, and the migration distance of plasma is greater in the re...

Embodiment 3

[0110] Next, a test piece was manufactured in the same manner as in Example 1 except that each reagent was used at the final concentration described in Table 3 below. In the same manner as in Example 2 above, the expected thickness of the reagent layer in each test piece was measured, and whole blood was spotted on the reagent layer, followed by measuring the migration distance of plasma in this case. Table 3 shows the reagent formulations for each test piece, and Table 4 shows the expected migration distance and measurement results of thickness.

[0111] Reagent

[0112] Reagent formula

[0113] From Tables 3 and 4, it can be seen that if the pH remains constant, the migration distance of plasma depends on the amount of particles used, that is, the thickness of the reagent layer, even if the amount of reagent used becomes larger or smaller.

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Abstract

A test strip having the capability of separating blood cells from whole blood, wherein it has a porous reagent layer comprising beads, an inorganic gel and a reagent that causes a detectable reaction with a substance to be detected, and a substrate that supports the reagent layer, wherein the beads are adhered to each other with the inorganic gel, and interstices are formed between the beads to trap a solid. The test strip can thus be used for separating blood cells from a whole blood including blood cells and plasma and detecting a substance to be detected, such as glucose, contained in plasma. The use of the test strip allows the measurement of a substance to be detected even by measurement of a transmitted light, the exhibition of good oxygen permeability, and thus can be used for measuring a substance to be detected with improved accuracy.

Description

technical field [0001] The invention relates to a test piece suitable for detecting a substance to be tested in blood, which mainly belongs to the field of chemical analysis of biological materials. Background technique [0002] When detecting any component contained in biological samples, such as blood, urine or cerebrospinal fluid, a series of analysis processes are usually required, such as absorption, diffusion, reaction, detection, etc. of the sample liquid. In particular, in a test using blood containing blood cells and plasma (hereinafter, sometimes referred to as "whole blood"), glucose in plasma is detected and quantified as a test substance, but blood cells may have various effects on the measurement results and tend to cause errors. For this reason, in blood tests using whole blood, we would like to be able to separate blood cells from plasma. Many tests have been performed to separate blood cells and plasma from whole blood and to detect test substances in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N31/22G01N21/78G01N21/86G01N33/48G01N33/50G01N33/52
CPCG01N21/78G01N33/5002G01N33/521G01N21/8483Y10T436/25375Y10T436/255
Inventor 平井香米原聪
Owner ARKRAY INC
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