Serum-free medium of human epidermis cell and its cultivating method and application

A technology of serum-free medium and epidermal cells, applied in artificial cell constructs, tissue culture, animal cells, etc., can solve the problem of growth rate not reaching the level of in vitro culture with serum, many unknown factors, and affecting the precise growth regulation mechanism of epidermal cells Research and other issues

Inactive Publication Date: 2003-07-23
江西医学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The traditional culture of epidermal cells is to add a certain proportion of serum to the culture medium. However, the composition of serum is very complex, there are many unknown factors, and the growth factors and hormones contained in each batch of serum vary greatly.
Therefore, the precise study of the mechanism of growth regu

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 A serum-free medium for human epidermal cells, including bovine serum albumin, human transferrin, insulin, cholesterol, catalase, and 0.01-10 micrograms / ml hydrocortisone.

Embodiment 2

[0031] Embodiment 2 A serum-free medium for human epidermal cells, including 80-120 mg / ml bovine serum albumin, 7-12 μg / ml human transferrin, 60-100 μg / ml insulin, 150-250 μg / ml Cholesterol, 150-250 micrograms / ml catalase, also includes 0.1-0.8 micrograms / ml hydrocortisone.

Embodiment 3

[0032] Embodiment 3 A serum-free medium for human epidermal cells, including 100 mg / ml bovine serum albumin, 10 μg / ml human transferrin, 80 μg / ml insulin, 200 μg / ml cholesterol, 200 μg / ml peroxide Hydrogenase, also includes hydrocortisone at 0.4-0.5 μg / ml. The serum-free medium is more suitable for the survival culture of human epidermal cells.

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PUM

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Abstract

A non-serum culture medium for human epidermal cells is based on IMDM culture medium and features additional use of hydrogen peroxidase, bovin serum albumin, human transferrin, cholesterin, insulin, hydrocortisone, epidermal cell growth factor, ox hypophysis extract and ethanolamine. Its advantages are no interference and high correctness and precision. It can be used for medicine research and medical purpose.

Description

technical field [0001] The invention relates to a cell culture medium and its culture method and application, in particular to a human epidermal cell serum-free medium and its application. Background technique [0002] The in vitro expansion and culture of human epidermal cells plays an important role in burn treatment, dermatology and pharmacology research. The traditional culture of epidermal cells is to add a certain proportion of serum to the culture medium. However, the composition of serum is very complex, with many unknown factors, and the growth factors and hormones contained in different batches of serum vary greatly. Therefore, traditional serum cultures affect the precise study of epidermal cell growth regulation mechanisms. Since the 1980s, foreign scholars have devoted themselves to exploring the possibility of serum-free culture of epidermal cells and achieved a certain degree of success, but they have not yet reached the growth rate of in vitro culture method...

Claims

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Application Information

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IPC IPC(8): C12N5/071
Inventor 陈国安袁利亚刘德伍辛国华戴育成
Owner 江西医学院
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