Microarray chip of oligonucleotide and its preparing method

A technology of oligonucleotides and microarray chips, which is applied in the field of preparation of low-cost oligonucleotide microarray chips, can solve the problems of limiting the popularization and application of oligonucleotide microarray chips and high preparation costs, etc. Achieve the effect of reducing preparation cost, avoiding high cost and high reliability

Inactive Publication Date: 2003-08-27
SOUTHEAST UNIV
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  • Abstract
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Problems solved by technology

Such high preparation costs greatly limit the populariza

Method used

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  • Microarray chip of oligonucleotide and its preparing method
  • Microarray chip of oligonucleotide and its preparing method
  • Microarray chip of oligonucleotide and its preparing method

Examples

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Example Embodiment

[0020] Example 1: refer to the attached figure 1 A preparation method of an oligonucleotide microarray chip. The details are as follows: 1. Synthesize a universal oligonucleotide 1 labeled with a special group (such as amino group or sulfhydryl group, etc.); 2. Synthesize an oligonucleotide probe 2 without any group modification. The needle consists of two parts (universal oligonucleotide complementary region 3 and probe specific region 4), and the probe specific region sequence is designed according to our needs; 3. The universal oligonucleotide 1 is mixed with different oligomers Nucleotide probe 2 is mixed in an equal proportion in a centrifuge tube (Eppendorf tube); 4. The above-mentioned Eppendorf tube is placed in boiling water for 2 to 5 minutes, and then naturally cooled to room temperature for renaturation. 5. Spotting the renatured product on the substrate 5 (such as aldehyde group or thiolated glass plate) that has been treated in advance through a spotting device ...

Example Embodiment

[0021] Example 2: refer to the attached figure 2 Another preparation method of oligonucleotide microarray chip. The details are as follows: 1. Synthesize a general oligonucleotide sequence 1 on a substrate 5 (such as a glass slide or other materials) using in situ synthesis technology; 2. Synthesize an oligonucleotide without any group modification Probe 2, the probe consists of two parts (universal oligonucleotide complementary sequence 3 and target gene complementary sequence 4), target gene complementary sequence 4 is designed according to our needs; 2. The nucleotide probes are spotted on the above-mentioned substrate to form a microarray chip; 4. The spotted chip is placed in a humidified hybridization box, and renatured at 42°C to 62°C for 4 hours; 5. Use 0.1% dodecane Unbound oligonucleotide probe 2 was removed by sodium alkyl sulfate (SDS), then washed thoroughly with double distilled water, and dried for later use.

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Abstract

The invention refers to a kind of oligopolynucleotide micro-array chip and the manufacturing method. The oligopolynucleotide probe is fixed on the base piece through a universal oligopolynucleotide segment and forms micro-array chip. The universal oligopolynucleotide chain may be embellished by special group (such as amido), then compounds with unnembellished oligopolynucleotide probe in centrifugal tube and forms double-chain. Finally it is fixed on specially worked glass piece or other material (such as aldehyde group) through sample applicator and forms the micro-array chip. Likewise, the universal oligopolynucleotide can be synthesized onto glass piece or other material through in situ synthesis technology, then uses the sample applicator to spot the unembellished oligopolynucleotide probe onto the glass piece or other material.

Description

technical field [0001] The invention relates to a method for preparing a low-cost oligonucleotide microarray chip. Through a common oligonucleotide chain, the oligonucleotide probe without any modification is immobilized on a glass slide or other materials to form a microarray chip. On the basis of retaining the high throughput, convenience, applicability and high reliability of the original chip, the technology greatly reduces the cost. technical background [0002] Gene chip is a high-tech developed rapidly in the field of life science in recent years. The so-called gene chip is a silicon chip, a glass slide, or a plastic chip on which a large number of gene probes or gene fragments are fixed in a specific arrangement. Gene chip technology is the main means to efficiently obtain relevant biological information on a large scale. At present, the application fields of this technology mainly include gene expression profile analysis, new gene discovery, gene mutation and pol...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 侯鹏陆祖宏祭美菊何农跃李松
Owner SOUTHEAST UNIV
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