HLA complement-dependent cytotoxcity antibody detection method using ELIA as basis and its kit
A kit and antibody technology, applied in the field of CDC effect evaluation, can solve problems such as insufficient satisfaction, and achieve the effect of simple and easy to master method, low cost and high sensitivity
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example 4 HLA-I or / and HLA-IIELISA approach ( 1 ( or )HLA-I or / and HLA-II。( 2 example 3 ( 3 、( 4 ( 3 1 ; or 1 。;, and 2-3, 1 。( 4 example 3 ( 8 、( 9 、( 10 。 example 5 HLA CFAbs approach (CFAbs-PRA)( 1 PRA preparation :HLA, 1 (0.01-3×106/);CELISA; or HLA or 。( 2 PRA 1 1 , and or ; example 3 ( 4 。( 3 or HLA;;;。( 4 HRP-C1qAb or HRP-C3Ab(), 1 ()。( 5 1 HRP,OD。( 6 :ODCFAbs;PRA 100 ;:CFAbs÷PRA×100=(PRA%) example :CFAbs25;PRA50;PRA%50%。 example 6 HLA-I and HLA-IICFAbs( 1 HLA-I or / and HLA-II or , example ,HLA-I or / and IICFAbs。( 2 HLA-ICFAbsHLA-I。( 3 HLA-I or II>95%HLA。 example 7
[0032] Or the total lgG on the surface of B lymphocytes can be measured to achieve simultaneous determination of CFABS and Non-CFAbs. Example 3: ELISA method for HLA Class I / IICFAbs (1) Purified HLA Class I or / and Class II antigens are coated with a microtiter plate at 37°C for 1-4 hours or 4°C overnight. Aspirate and discard the package. By liquid. (2) Block the microplate with a buffer (blocking solution) containing a certain amount of non-specific protein (such as BSA, skimmed milk powder, calf serum, etc.), with the same time and temperature as step (1), and then aspirate the remaining blocking liquid. (3) Add 50 μl each of negative control serum, positive control serum, background buffer and test serum into the corresponding microplates (also can be serum diluted with buffer). (4) Incubate the above-mentioned microplate at 37°C or 4°C for 30 minutes to 3 hours or 4°C overnight. (5) Aspirate and discard the reaction solution, and wash each well of the microplate with a deterge...
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