Plant root excretion continuous collection method and apparatus

A technology of root exudates and exudates, applied in the field of continuous collection methods and devices for plant root exudates, capable of solving problems such as low exudate content, denaturation of compounds, prolonging collection time, etc., and achieving the effect of short collection time

Inactive Publication Date: 2004-03-17
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Today, however, our knowledge of rhizosphere chemistry is extremely limited, mainly because researchers often face the following three problems when studying rhizosphere exudates: The exudates to be collected are usually very low in content, requiring a large number of plants and prolong the collection time to obtain a sufficient amount of secretions; the collection and processing of samples is very cumbersome, and some unstable compounds will be denatured; when the traditional solvent extraction method collects trace secretions, growth substrates, containers, nutrient solutions, water , extractant, etc. will cause serious contamination to the sample
Although the rhizosphere soil sol

Method used

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  • Plant root excretion continuous collection method and apparatus
  • Plant root excretion continuous collection method and apparatus

Examples

Experimental program
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Effect test

Embodiment 1

[0017] [Example 1] Provide the nutrients needed for plant growth with Hoagland nutrient solution of 1 / 3 strength, after the plants grow to a certain period (about 20 days), rinse with distilled water in the bottomless glass dish Quartz sand 4 times (200ml each time), and then washed 2 times with deionized water (18.2MΩ2cm). Install the glass bottle containing Amberlite IRC-50 cationic resin and other devices, connect the power supply, and fill the air pump with continuous air bubbles, which will make the solution circulate through the resin in the glass bottle, and the resin will adsorb amino acids. After 60-72 hours of collection, the solution in the glass bottle was sucked dry, eluted, concentrated and filtered. Amino acids were determined with or without derivatization.

Embodiment 2

[0018] [Example 2] Provide the nutrient elements needed for the plant growth of inoculated mycorrhizal fungi with 1 / 3 strength Hoagland (Hoagland) nutrient solution, after the plant grows to a certain period (about 20 days), rinse with distilled water Put the quartz sand in the bottomless glass dish 4 times (200ml each time), and then wash it with deionized water (18.2MΩcm) twice. Install the glass bottle containing Amberlite IRC-50 cationic resin and other devices, connect the power supply, and fill the air pump with continuous air bubbles, which will make the solution circulate through the resin in the glass bottle, and the resin will adsorb amino acids. After 60-72 hours of collection, the solution in the glass bottle was sucked dry, eluted, concentrated and filtered. Amino acids were determined with or without derivatization.

Embodiment 3

[0019] [Example 3] Provide the nutrient elements needed for plant growth with 1 / 3 strength Hoagland (Hoagland) nutrient solution, after the plant grows to a certain period (about 20 days), rinse in the bottomless glass dish with distilled water Quartz sand 4 times (200ml each time), and then washed with deionized water (18.2MΩcm) 2 times (200ml each time). Install the glass bottle containing Dowex-1 anion resin, connect the power supply, and fill the air pump with continuous air bubbles. The air bubbles will make the solution circulate through the resin in the glass bottle, and the resin will absorb the organic acid. After 60-72 hours of collection, the solution in the glass bottle was sucked dry, eluted, concentrated and filtered. Determination of organic acids.

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Abstract

A plant root excretion continuous collection method and apparatus, wherein the plant is placed in the cultivation dish equipped with quartz sand, the bottom opening of the cultivation dish is connected to a glass bottle containing resin through pipe lines, an opening is arranged at the bottom of the glass bottle, then connected to the cultivation dish through pipe lines, forming a circulated system, adding different solvents into the circulating pipes, for the three dissimilar collected root secretion, i.e., neutral, acidic or basic, making the root secretion absorbed on the surface of the resin when flowing through the resin along with the solvent of the circulatory flow, after a specific period of time, perform elution, compression and filtration to the root secretion, finally conduct measuring.

Description

1. Technical field [0001] The invention relates to the collection of plant root exudates, in particular to a method for continuously collecting plant root exudates and a device thereof, belonging to the fields of soil, microorganism and plant science. 2. Background technology [0002] In addition to the shed cells and the mucus produced, plant roots also secrete a wide variety of soluble substances, such as sugars with different molecular weights, organic acids, amino acids, fatty acids, steroids, enzymes, auxins, etc., mainly Sugars, organic acids and amino acids. Root exudates vary widely by plant species, physiological factors (e.g., age, nutritional status) and abiotic conditions (e.g., temperature, soil structure, aeration, and moisture content), and are also related to microbial activity (amount consumed), from root The amount that is secreted and then enters the root is related to the damage degree of the root, and if it is collected in the soil, it is related to the...

Claims

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Application Information

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IPC IPC(8): G01N1/02G01N1/28
Inventor 廖继佩林先贵曹志洪
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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