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Construction process of rice genetic test material

A technology for rice and japonica rice, which is applied in the field of biology and achieves the effects of fully separating main effects and interaction effects, uniform and consistent plant shape, and improving accuracy.

Inactive Publication Date: 2004-04-07
SHANGHAI AGROBIOLOGICAL GENE CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially when it comes to accurately tracking the gene flow between subspecies, studying the gene interaction between rice subspecies, and analyzing the different types of gene redundancy caused by the use of foreign germplasm, the above-mentioned traditional genetic materials are difficult to obtain. ideal result

Method used

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  • Construction process of rice genetic test material
  • Construction process of rice genetic test material
  • Construction process of rice genetic test material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1, a method for constructing rice genetic test materials in the present invention is to construct isogenic two-way introduction lines of indica rice background and japonica rice background, wherein: construction of two-way introduction lines: select the indica-type high-yield varieties Teqingheyuan originally produced in my country The high-quality japonica rice variety Lemont produced in the United States was used as the parent, and the F1 generation was obtained by cross-crossing using techniques well known to those skilled in the art; Teqing and Lemont were used as recurrent parents, respectively, through 3 backcrosses and more than 2 self-crosses. Results: More than 200 bidirectional introductory lines were obtained, the populations had extensive phenotypic variation, the introductory effect was obvious, and the introductory fragments covered the entire linkage map.

Embodiment 2

[0022] Embodiment 2, the detection of the phenotype of the indica rice background introduction line

[0023] The phenotypes of indica rice background introduction lines were identified by random block three-repetition experiment, and their growth period, plant height, leaf shape, tiller leaf angle, number of panicles, panicle length, number of branches, number of spikelets, grain density, and seed setting rate were identified. , 100-grain weight, and grain yield characteristics were observed and determined. The results showed that the indica rice introduced line population had extensive phenotypic variation, the introduction effect was obvious, and the plant morphology was relatively neat.

[0024] Genotype detection: 146 molecular markers and morphological markers were used to detect the genotypes of all strains, and a detailed analysis of the imported components of different strains and marker sites, such as figure 1 The structure diagram of the isogenic introduction line o...

Embodiment 3

[0025] Example 3, detection of phenotypes of japonica rice background introduction lines

[0026] The phenotypes of japonica rice background introduction lines were identified by random block three-repeat test, and their growth period, plant height, leaf shape, tiller leaf angle, number of panicles, panicle length, number of branches, number of spikelets, grain density, and seed setting rate , 100-grain weight, and grain yield characteristics were observed and determined. The results showed that the japonica rice introductory line population had extensive phenotypic variation, the introductory effect was obvious, and the plant morphology was relatively uniform. The genotypes of all strains were detected by 139 molecular markers and morphological markers, and detailed analysis of imported components was carried out on different strains and marker sites, such as figure 2 , image 3 , the structure diagram of the isogenic introduction line of the Lemont background, in which P1...

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Abstract

The present invention relates to genetic rice breeding method and biotechnological research method. The construction process of rice genetic test material includes hybridizing indica rice variety and japonica rice variety to obatin F1 generation; thrice backcross with the said indica rice variety or the japonica rice variety as recurrent parent; and selfing for 2-8 times to obtain bi-directional isogenic introduction line with indica rice and japonica rice background. Compared with other genetic test materials and molecular locating colonies, the introduction line of the present invention has indica rice and japonica rice background, and each strain line has a few introduced segments and relatively regular plant form. The present invention may be used in genetic test, breeding, ect. and the introduction line colony has raised phynotype identification and number and characteristic locating precision in rice gene location, fully separated main effect from interaction effect and may produce active effect in map position cloning and other application.

Description

technical field [0001] The invention relates to a method for constructing rice genetic testing materials and relates to the field of biology. The method of constructing rice genetic test material is a method of rice genetic breeding and biotechnology research method. The isogenic two-way introduction line of indica rice background and japonica rice background is constructed by hybridization, which can be used for genetic testing and breeding. Background technique [0002] Rice genetics and breeding research began at the beginning of the last century. In the past hundred years, scientists have conducted a lot of research on the morphology, physiology and reproductive traits of rice, and have made significant progress in both cytogenetics and molecular genetics of rice. In these studies, genetic test materials as the material basis play a very important role. However, with the rapid development of modern biotechnology and the in-depth study of rice genetics, previous genetic...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 罗利军梅捍卫余新桥黎志康
Owner SHANGHAI AGROBIOLOGICAL GENE CENT
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