Figwort polysaccharide and its production process and use

A production method and technology of Scrophulariaceae, applied in the field of homologous polysaccharide mixtures, to achieve high yield and simple extraction and separation methods

Inactive Publication Date: 2004-04-07
SHANGHAI INST OF ORGANIC CHEMISTRY - CHINESE ACAD OF SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Literature reports, [Zhang Wenjie et al., Yunnan Botanical Research, 16(4), 407(1994)], [Li Yiming, Jiang Shanhao, Gao Wenyun, Zhu Dayuan, Acta Pharmaceutica Sinica, 34(6), 448(1999)], [Zou Chenting, Yang Xiuwei, Chinese Herbal Medicine, 31(4), 241(20

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  • Figwort polysaccharide and its production process and use
  • Figwort polysaccharide and its production process and use
  • Figwort polysaccharide and its production process and use

Examples

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[0030] Example 1

[0031] Wash and dry 500g of the crude drug Scrophulariaceae, pulverize, soak in 5 liters of deionized water at room temperature for 24 hours, and filter. The residue was continuously immersed in 2.5 liters of deionized water for 12 hours, filtered, and the filtrate was combined and concentrated under reduced pressure at 60°C to 1-2 liters. Then it was precipitated with 95% ethanol, the final concentration of ethanol was 80%, and the precipitation was carried out for 24 hours. Centrifuge, dissolve the precipitate with 1.25 liters of water, centrifuge, and dialyze in ion-free water for 48 to 72 hours. After concentration under reduced pressure at 60° C. and freeze-drying, 8.9 g of total sugars of Scrophulariae are obtained. Add 2 liters of water to dissolve, add activated carbon treatment, repeat the removal three times, then concentrate under reduced pressure, centrifuge and freeze-dry to obtain 3.0 g of total sugar of Scrophulariaceae wi...

Example Embodiment

[0032] Example 2

[0033]Wash 500g of the crude drug Scrophulariaceae, dry, crush, soak in 5 liters of deionized water at room temperature for 36 hours, and filter. The residue was soaked in 2.5 liters of deionized water for 12 hours, filtered, and the filtrate was combined and concentrated under reduced pressure at 50°C to 2 liters. Then it was precipitated with 95% ethanol, the final concentration of ethanol was 82%, and the precipitation was 48 hours. After centrifugation, the precipitate was dissolved in 2 liters of water, centrifuged, and dialyzed against ion-free water for 72 hours. After concentration under reduced pressure at 50°C and freeze-drying, 9.5 g of total polysaccharides of Scrophularia cepacia was obtained. Add 2 liters of water to dissolve, add activated carbon treatment, repeat the removal three times, then concentrate under reduced pressure, centrifuge and freeze-dry to obtain 3.1 g of total polysaccharides of Scrophulariaceae with a p...

Example Embodiment

[0034] Example 3

[0035] Scrophulariaceae total sugars are refined by DEAE-Cellulose column chromatography with a purity of 85-90%, and then Sephadex G-25 and Sephadex G-75 column chromatography are used to obtain Scrophulariaceae polysaccharide SnPS-1 with a purity of 99.6%. The specific separation method: weigh 100 mg of the total sugar of Scrophulariaceae, dissolve it in 1-2 mL of distilled water, and centrifuge (4500r / min, 10min). Take the supernatant and apply it to a Sephadex G-25 column (1.5cm×100cm), eluting with 0.1mol / L NaCl, at a flow rate of 0.5mL / min, and collect 3-5mL per tube. About 37-47 tubes are the absorption peak. After collection, it was purified by Sephadex G-75 column and freeze-dried to obtain pure polysaccharide SnPS-150mg.

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Abstract

The present invention is polysaccharide extracted from Chinese medicinal material figwort and its production process and use. Figwort is processed to obtain total saccharide through water soaking, organic solvent precipitation, water dissolution of the precipitate, dialysis of supernatant or membrane separation, concentration and drying; and figwort total saccharide is further processed through active carbon treatment and column chrumatography to obtain figwort polysaccharide SnPS-1. The chemical structure of SnPS-1 includes main chainsí·2)Gal(1í·and í·2)Araf(1í·; small amount of sacharide chains í·6) Manp (1í·, í·5) Araf(1í·, í·3) Glcp (1í· and í·3) Rhap (1í·; thimbleful branch saccharide í·2ú¼4ú® Xylp(1í·; and terminal saccharide Galp (1í·, and this shows that SnPS-1 is heteropolysaccharide with straight chain as main component and less branches. Bioactivity experimant shows that the polysaccharide has cerebral ischemica protecting function and no toxic side effect.

Description

technical field [0001] The invention relates to a polysaccharide homologous mixture extracted from Chinese medicinal materials, in particular to Scrophulariaceae polysaccharide SnPS-1 extracted from crude drug Scrophulariaceae Scrophulariae, its production method and application. technical background [0002] Scrophulariaceae is a traditional Chinese medicinal material, which has the effects of "cooling blood and nourishing yin, reducing fire, promoting body fluid, and detoxifying". Literature reports, [Zhang Wenjie et al., Yunnan Botanical Research, 16(4), 407(1994)], [Li Yiming, Jiang Shanhao, Gao Wenyun, Zhu Dayuan, Acta Pharmaceutica Sinica, 34(6), 448(1999)], [Zou Chenting, Yang Xiuwei, Chinese Herbal Medicine, 31(4), 241(2000)], its chemical components mainly include alkaloids, flavonoid glycosides, sterols, amino acids, fatty acids, volatile oils, carotene, iridoid glycosides and trace elements, etc. Studies on polysaccharides in Scrophulariaceae have not been report...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61P9/10C08B37/00
Inventor 田庚元邓军娥
Owner SHANGHAI INST OF ORGANIC CHEMISTRY - CHINESE ACAD OF SCI
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