Method and kit for testing resistivity of insects to pesticides

Abstract

A method for checking for sensitivity of acetylcholine esterase in insects using a single insect, which comprises the steps, in any order, of homogenizing a single insect in aqueous neutral to acid pH phosphate buffer, producing a solution of acetylcholine iodide in neutral to acid pH phosphate buffered water-miscible organic solvent, producing a solution of 5,5-dithio-bis-(2-nitrobenzoic acid) in the buffer, and producing a solution of propoxur in the buffer, dropping the insect homogenisate into the wells of an assay plate, dropping the acetylcholine iodide and 5,5-dithio-bis-(2-nitrobenzoic acid) solutions into some of the wells and 5,5-dithio-bis-(2-nitrobenzoic acid) propoxur solutions into the other wells and checking for a difference in the yellow coloration between the samples in the two sets of cells. The invention also provides a test kit for determining susceptibility of acetylcholine esterase in insects using a single insect comprising a container containing separate containers of: I. a neutral to acid pH phosphate buffer II. acetylcholine iodide III. 5,5-dithio-bis-(2-nitrobenzoic acid) IV. propoxur and V. a water-miscible organic solvent.

Description

technical field [0001] The present invention relates to methods and kits for determining the resistance of insects to insecticides, and more particularly provides methods and kits for use in the field to determine resistance of insects to insecticides containing organophosphates and carbamates. Background technique [0002] Chemical insecticides are still currently the most important agents in the control of disease vectors such as malaria, dengue fever, filariasis, etc. Since the invention of the first synthetic insecticide DDT in 1940, the number of deaths from the spread of these diseases has been greatly reduced. However, the continued use of insecticides leads to the development of resistance of vectors carrying the above-mentioned diseases, mainly insects, which reduces the effectiveness of the control vectors and thus contributes to the spread of diseases. The determination of resistance is mainly based on the standard test methods of the World Health Organization (W...

AI Technical Summary

Problems solved by technology

However, the continued use of insecticides has led to the development of resistance to vectors carrying the above-mentioned diseases, mainly insects, which reduce the effectiveness of the control vectors and thus contribute to the spread of diseases

The determination of resistance is mainly based on the standard test methods of the World Health Organization (WHO). These methods are particularly difficult for users to use, take a long time, require many insect samples, use kits with short shelf life and require Skilled personnel conduct tests and interpret test results

As a result, the level of susceptibility of many insect vectors is generally unknown, and insect susceptibility can affect control outcomes