Method of preoceeding qualitative and/or quantitative analysis against target substance in sample and its detecting device

A quantitative analysis and target technology, applied in the direction of material analysis by optical means, analysis by chemical reaction of materials, measurement devices, etc., can solve the problems of reducing detection cost, low sensitivity, and high cost of activated film base

Inactive Publication Date: 2004-07-21
CHENGDU KUACHANG SCI & TECH CO LTD
View PDF1 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current composite light irradiation detection method based on a diffusible polymer membrane base is not very sensitive; the current chemiluminescence detection method is not very sensitive; the current fluorescence detection method is more sensitive than the former two However, there are still dis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: fluorescent body detection method and black biochip

[0045] In this example, the biochip used is a black biochip containing a black film base, the colorant used is rhodamine, and the marker containing the colorant used is a rhodamine-labeled goat anti-human secondary antibody (JacksonImmunoRresearch Laboratories, USA). The reference photobase is a transparent amino-modified glass slide with a size of 75×25×1 mm (Telechem International, USA, hereinafter referred to as substrate 0).

[0046] The steps of this embodiment are as follows:

[0047] - Preparation of black film base:

[0048] The black background film bases prepared in this example are: a unitary black material base, a black coating-structure film base, a black membrane-structure film base and a transparent-black material composite film base.

[0049] The monolithic black material sheet base prepared in this embodiment is a black plastic sheet base, which is made of thermoplastic polyvinyl chlo...

Embodiment 2

[0063] Example 2: Fluorescent body detection method and black "rough" biochip

[0064] In this example, the biochip used is a black "rough" biochip containing a black "rough" film base, and the colorants, markers containing colorants, and reference photobase 0 used are the same as in Example 1.

[0065] The steps of this embodiment are as follows:

[0066] The black "rough" film base prepared in this example is a coating-structure film base. The coating is formed by black paint, and the structural piece is a transparent glass slide (Erie Scientific Company, USA) with a size of 75×25×1 mm. The black paint used in this embodiment is an automobile finish (pearl black, Shanghai Qifu Industrial Development Co., Ltd.). Spray black paint on the top surface of the glass slide, and form a black coating (thickness is about 30um) after drying, which is recorded as the film base 21. The roughness of the base 21 is (detected by the Metrology Supervision and Inspection Test of Chengdu Ci...

Embodiment 3

[0072] Embodiment 3: Composite light body detection method and white biochip

[0073] In this example, the biochip prepared and used is a white biochip containing a white base, and the reference base 0 used is the same as that in Example 1.

[0074] The steps of this embodiment are as follows:

[0075] - Preparation of white film base:

[0076] The white background film bases prepared in this example are: unitary white material film bases, white coating-structure film bases and transparent-white material composite film bases.

[0077] The unitary white material sheet base prepared in this example is a white plastic sheet base, which is made of thermoplastic polyvinyl chloride after adding fillers such as titanium dioxide, and then molded. The size is 75×25×1mm, hereinafter referred to as sheet Base 31.

[0078] The white coating-structure sheet base prepared in this embodiment, the coating is formed by white paint, and the structure sheet is a transparent glass slide (Erie ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
The average particle sizeaaaaaaaaaa
Average particle sizeaaaaaaaaaa
Roughnessaaaaaaaaaa
Login to view more

Abstract

The present invention discloses a method which uses coloured biological chip to carry on qualitative and/or quantitative analysis for sample, especially for target object in biological sample and device to be used in the method. In the method, optimum program is selected to generate maximization contrast for increasing possibility to raise analysis sensitivity, enlarging much freedom for chip base selection and scanner selection in order to realize quite high detection sensitivity with quite low cost by utilizing obvious contrast of brightness, saturation and signal light colour difference formed between coloured background and coloured target on chip.

Description

technical field [0001] The invention relates to a detection method and a detection device for qualitatively and / or quantitatively analyzing target objects in samples, especially biological samples. Background technique [0002] There are a wide range of methods for qualitative and / or quantitative analysis of targets in samples, especially biological samples, among which the basic principle of a class based on probe-target specific reactions is: make the probe and the sample The target molecule in the system performs a specific reaction, and then analyzes the result of this specific reaction. The basic steps of this type of analysis method are: preparing a device containing a probe reactor, bringing a sample into contact with the reactor and performing a relevant reaction, and analyzing the reaction result. Due to the different probe reactor devices used, many qualitative and / or quantitative analysis methods have been derived, for example, biochip method, PCR method, rapid d...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/00G01N21/64G01N21/76G01N33/00G01N33/48G01N33/50G01N33/53G01N33/543G01N33/68
CPCG01N33/54346
Inventor 邹方霖陈春生王建霞陈宁
Owner CHENGDU KUACHANG SCI & TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap